The mechanism of Naringin-enhanced remyelination after spinal cord injury
| Autor: | Fei Song, Xu Cai, Cheng Zhang, Song-Hua Xiao, Yongwei Pan, Zhe Zhao, Wei Rong |
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| Rok vydání: | 2017 |
| Předmět: |
3'-cyclic nucleotide 3'phodiesterase
behavioral assessment neural regeneration 0301 basic medicine medicine.medical_specialty oligodendrocytes oligodendrocyte precursor cells nerve regeneration spinal cord injury naringin remyelination β-catenin glycogen synthase kinase-3β NKx2.2 2' lcsh:RC346-429 White matter 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience Internal medicine medicine Remyelination Glycogen synthase Spinal cord injury Naringin lcsh:Neurology. Diseases of the nervous system biology business.industry 2’ 3’-cyclic nucleotide 3’phodiesterase Phosphodiesterase medicine.disease Spinal cord 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry biology.protein Signal transduction business Neuroscience 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 12, Iss 3, Pp 470-477 (2017) |
| ISSN: | 1673-5374 |
| Popis: | Our previous study revealed that intragastric administration of naringin improved remyelination in rats with spinal cord injury and promoted the recovery of neurological function of the injured spinal cord. This study sought to reveal the mechanisms by which naringin improves oligodendrocyte precursor cell differentiation and maturation, and promotes remyelination. Spinal cord injury was induced in rats by the weight-drop method. Naringin was intragastrically administered daily (20, 40 mg/kg) for 4 weeks after spinal cord injury induction. Behavioral assessment, histopathological staining, immunofluorescence spectroscopy, ultrastructural analysis and biochemical assays were employed. Naringin treatment remarkably mitigated demyelination in the white matter, increased the quality of myelinated nerve fibers and myelin sheath thickness, promoted oligodendrocyte precursor cell differentiation by upregulating the expression of NKx2.2 and 2′3′-cyclic nucleotide 3′-phosphodiesterase, and inhibited β-catenin expression and glycogen synthase kinase-3β (GSK-3β) phosphorylation. These findings indicate that naringin treatment regulates oligodendrocyte precursor cell differentiation and promotes remyelination after spinal cord injury through the β-catenin/GSK-3β signaling pathway. |
| Databáze: | OpenAIRE |
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