Molecular Epidemiological Traits of Group A Rotaviruses in Japanese Children During Transitional Period of Rotavirus Vaccine Implementation, 2011 - 2014
| Autor: | Satoshi Hayakawa, Sayaka Takanashi, Kumiko Sugita, Hiroshi Ushijima, Shoko Okitsu, Masaaki Kobayashi, Masashi Mizuguchi, Hideaki Kikuta, Shuichi Nishimura, Aksara Thongprachum, Tsuneyoshi Baba, Atsuko Yamamoto |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Rotavirus
0301 basic medicine medicine.medical_specialty Genotype 030106 microbiology Biology medicine.disease_cause Group A Rotavirus Infections General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Japan Epidemiology medicine Humans 030212 general & internal medicine Child Genotyping Phylogeny Molecular Epidemiology Rotavirus Vaccines Rotavirus vaccine Virology Group A rotaviruses Detection rate |
| Zdroj: | Clinical Laboratory. 63 |
| ISSN: | 1433-6510 |
| Popis: | BACKGROUND Group A rotavirus (RVA) vaccines have been introduced in Japan since 2011. To investigate the molecular epidemiological traits of RVA during the transitional period of rotavirus vaccine implementation in Japan, this study was undertaken by following up three-decade long surveillance conducted in the same regions. METHODS RVA were screened and genotyped by RT-PCR from diarrheal samples collected from non-hospitalized patients in six localities (Hokkaido, Tokyo, Shizuoka, Osaka, Kyoto, and Saga) during 2011 - 2014. Selected samples were sequenced to elucidate the evolutionary trend. RESULTS Among 1858 specimens, the detection rate of RVA declined to 4.0% in 2013 - 2014 from 17.9% in 2011 - 2012 and 22.1% in 2012 - 2013. G1P[8] was the most predominant genotype in the first two years accounting for more than half, and G9P[8] showed the highest detection rate as 35.0% in the last year. Interestingly, the proportional rate of G2 strains in the studied period increased from 0% to 25%. VP6 genotyping revealed that DS-1 like reassortant G1P[8] strains were detected all over Japan and their prevalence fluctuated greatly from 35.0% to 89.5%. Sequence analysis of VP6 showed that strains in the current strains were closely related but distinct from the original reference strains, namely Wa and DS-1. CONCLUSIONS The detection rates of RVA, their GP combinations, prevalence of reassortant strains varied greatly after the introduction of rotavirus vaccines in Japan. Continuous monitoring is warranted to refine future vaccine strategy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|