Enhanced flavour formation of combination of selected lactococci from industrial and artisanal origin with focus on completion of a metabolic pathway
| Autor: | Gerrit Smit, Annette Verheul, Jan T.M. Wouters, W.J.M. Engels, Eman H.E Ayad |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cell Extracts
Fermentation starter Carboxy-Lyases Lactococcus Proteolysis Flavour Cheese ripening Applied Microbiology and Biotechnology Leucine medicine Animals Life Science Food science Amino Acids Flavor Transaminases VLAG chemistry.chemical_classification Aldehydes Cacao biology medicine.diagnostic_test Leerstoelgroep Productontwerpen en kwaliteitskunde General Medicine Product Design and Quality Management Group biology.organism_classification Milk Proteins Keto Acids Amino acid Lactococcus lactis Metabolic pathway Milk chemistry Biochemistry Taste Cattle Biotechnology |
| Zdroj: | Journal of Applied Microbiology 90 (2001) Journal of Applied Microbiology, 90, 59-67 |
| ISSN: | 1364-5072 |
| Popis: | E.H.E. AYAD, A. VERHEUL, W.J.M. ENGELS, J.T.M. WOUTERS AND G. SMIT. 2001. Combinations of lactococcal strains from various origins with divers properties were developed as new starters for new dairy products. Flavour formation by such tailor-made cultures was studied. In some cases, a strongly enhanced flavour was observed. For instance, the combination of B1157 and SK110 strains in milk resulted in a very strong chocolate-like flavour. B1157 produces only a moderate chocolate-like flavour, whereas SK110 alone fails to produce this flavour. Headspace gas chromatography results corroborate the organoleptic evaluations. High levels of branched-chain aldehydes were found when B1157 and SK110 were grown together. The enzyme activities involved in this pathway were studied; both strains contain transaminase activity. Although B1157 had a very high amino acid decarboxylating activity, its release of amino acids from milk protein was limited. SK110 was strongly limited in decarboxylating activity, although this strain is very active in proteolysis. By combining these strains, the substrates released by SK110 can directly be used by the other strain, resulting in the completion of the whole flavour-formation pathway. This opens new avenues for the preparation of tailor-made cultures. |
| Databáze: | OpenAIRE |
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