Maintenance of the marginal-zone B cell compartment specifically requires the RNA-binding protein ZFP36L1
| Autor: | Newman, R, Ahlfors, H, Saveliev, A, Galloway, A, Hodson, DJ, Williams, R, Besra, GS, Cook, CN, Cunningham, AF, Bell, SE, Turner, M |
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| Přispěvatelé: | Hodson, Daniel [0000-0001-6225-2033], Bell, Sarah Elizabeth [0000-0002-3249-707X], Turner, Martin [0000-0002-3801-9896], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
B-Lymphocytes
Lymphoid Tissue Sequence Analysis RNA Kruppel-Like Transcription Factors Fluorescent Antibody Technique High-Throughput Nucleotide Sequencing Nuclear Proteins RNA-Binding Proteins Enzyme-Linked Immunosorbent Assay Flow Cytometry Real-Time Polymerase Chain Reaction Mice Phenotype Gene Expression Regulation Cell Movement Interferon Regulatory Factors Cell Adhesion Animals Butyrate Response Factor 1 Signal Transduction |
| Popis: | RNA-binding proteins of the ZFP36 family are best known for inhibiting the expression of cytokines through binding to AU-rich elements in the 3' untranslated region and promoting mRNA decay. Here we identified an indispensable role for ZFP36L1 as the regulator of a post-transcriptional hub that determined the identity of marginal-zone B cells by promoting their proper localization and survival. ZFP36L1 controlled a gene-expression program related to signaling, cell adhesion and locomotion; it achieved this in part by limiting expression of the transcription factors KLF2 and IRF8, which are known to enforce the follicular B cell phenotype. These mechanisms emphasize the importance of integrating transcriptional and post-transcriptional processes by RNA-binding proteins for maintaining cellular identity among closely related cell types. |
| Databáze: | OpenAIRE |
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