Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications
| Autor: | Huizhen Zheng, Ruyun Lou, Meng Gao, Yizhe Song, Xin Guo, Ying Ren, Xiaojun Ma, Xiudong Liu, Hongguo Xie, Weiting Yu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Alginates Cell Survival Asialoglycoproteins Capsules 02 engineering and technology Biochemistry Permeability Rats Sprague-Dawley Chitosan 03 medical and health sciences chemistry.chemical_compound Glucuronic Acid Structural Biology medicine Animals Organic chemistry Polylysine Molecular Biology Mechanical Phenomena Hexuronic Acids Galactose Water Biological Transport General Medicine 021001 nanoscience & nanotechnology Lactobionic acid Rats Molecular Weight carbohydrates (lipids) 030104 developmental biology medicine.anatomical_structure Solubility chemistry Chemical engineering Covalent bond Permeability (electromagnetism) Hepatocyte Hepatocytes Urea Asialoglycoprotein receptor 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 93:1133-1140 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2016.09.078 |
| Popis: | Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte-matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect