Retinal glial cell immunoreactivity and neuronal cell changes in rats with STZ-induced diabetes
| Autor: | Carl-David Agardh, Elisabet Agardh, Anitha Bruun |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
cis-trans-Isomerases Calbindins medicine.medical_specialty Normal diet Probucol Cell Count Guanidines Calbindin Antioxidants Retina Streptozocin Diabetes Mellitus Experimental Cellular and Molecular Neuroscience chemistry.chemical_compound S100 Calcium Binding Protein G Internal medicine Glial Fibrillary Acidic Protein medicine Animals Enzyme Inhibitors Rats Wistar Eye Proteins Fluorescent Antibody Technique Indirect Pigment Epithelium of Eye Ganglion cell layer Neurons Diabetic Retinopathy biology Proteins RNA-Binding Proteins Retinal Primary and secondary antibodies Sensory Systems Rats Ganglion Ophthalmology medicine.anatomical_structure Endocrinology ELAV Proteins chemistry Cis-trans-Isomerases biology.protein sense organs Carrier Proteins Neuroglia medicine.drug |
| Zdroj: | Current Eye Research. 23:276-284 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1076/ceyr.23.4.276.5459 |
| Popis: | To study whether diabetes could influence glial cells, retinal neurons, and pigment epithelial cells and if so, to evaluate whether any changes could be influenced by aminoguanidine (AG) or probucol (PB).Streptozocin (STZ)-induced diabetic male Wistar rats and age-matched control rats were fed a normal diet, addition of AG in the drinking water (0.5 g/l for diabetic and 1.0 g/l for control rats) or PB in the pellets (1 % w/w) for one or six months. Paraffin embedded retinal sections were incubated in the primary antibodies GFAP, calbindin, RPE65, and Hu, for glial, horizontal, pigment epithelial, and ganglion cells, respectively, and in fluorescent secondary antibodies.One month after STZ injection, GFAP immunoreactivity was sparse, but after six months it was prominent in glial cells in 5/5 diabetic and 1/7 control retinas (p = 0.015). Neither AG, nor PB influenced this immunoreactivity. Numbers of retinal pigment epithelial cells and cells in the ganglion cell layer, were similar at one and six months of diabetes. By time, the number of horizontal cells decreased (p0.001) and branching and numbers of their terminals were reduced (p0.001).Diabetes for six months resulted in increased glial cell immunoreactivity, and by age, horizontal cell numbers and branching of their terminals decreased, morphological patterns that were unaffected by AG or PB. The numbers of retinal pigment epithelial cells and cells in the ganglion cell layer were unaffected both by age and diabetes. |
| Databáze: | OpenAIRE |
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