The mouse median nerve experimental model in regenerative research
| Autor: | Stefano Geuna, Sara Buskbjerg Jager, Giulia Ronchi, Christian Bjerggaard Vaegter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Genetically modified mouse
Pathology medicine.medical_specialty Contraction (grammar) Article Subject lcsh:Medicine General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences Myelin Mice 0302 clinical medicine law Peripheral Nerve Injuries Hand strength medicine Animals Humans Muscle Skeletal 030304 developmental biology 0303 health sciences General Immunology and Microbiology Hand Strength Chemistry lcsh:R General Medicine Anatomy Median nerve Median Nerve Nerve Regeneration Disease Models Animal medicine.anatomical_structure Sciatic nerve Electron microscope Epineurial repair 030217 neurology & neurosurgery Research Article |
| Zdroj: | Buskbjerg Jager, S, Ronchi, G, Vægter, C B & Geuna, S 2014, ' The mouse median nerve experimental model in regenerative research ', BioMed Research International, vol. 2014, 701682 . https://doi.org/10.1155/2014/701682 BioMed Research International BioMed Research International, Vol 2014 (2014) |
| DOI: | 10.1155/2014/701682 |
| Popis: | Sciatic nerve crush injury in rat animal model is one of the most common experimental models used in regenerative research. However, the availability of transgenic mouse for nerve regeneration studies is constantly increasing and, therefore, the shift from rat model to mouse model is, in some cases, necessary. Moreover, since most of the human nerve lesions occur in the upper limb, it is also advantageous to shift from sciatic nerve to median nerve. In this study we described an experimental model which involves lesions of the median nerve in the mouse. Data showed that the finger flexor muscle contraction strength, assessed to evaluate the motor function recovery, and reached values not different from the control already 20 days after injury. The degree of nerve regeneration evaluated with stereological methods in light microscopy showed that, 25 days after injury, the number of regenerated myelinated fibers was comparable to the control, but they were smaller with a thinner myelin thickness. Stereological analysis made in electron microscopy confirmed these results, although the total number of fibers quantified was significantly higher compared to light microscopy analysis, due to the very small size of some fibers that can be detected only in electron microscopy. |
| Databáze: | OpenAIRE |
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