Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments
| Autor: | Alvar Trucco, Alberto Luini, Massimiliano Baldassarre, Willie J. C. Geerts, Roberto Buccione, Roman S. Polishchuk, Alexander A. Mironov, Enrica San Pietro, Abraham J. Koster, Daniele Di Giandomenico, Galina V. Beznoussenko, Oliviano Martella, Aurora Fusella, Koert N.J. Burger, Alessio Di Pentima, Elena V. Polishchuk |
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| Rok vydání: | 2004 |
| Předmět: |
PROTEINS
Vesicle Endoplasmic reticulum ENDOPLASMIC-RETICULUM Golgi Apparatus Biological Transport Cell Biology APPARATUS Biology Golgi apparatus Endoplasmic Reticulum Cell biology Cell Compartmentation Cell Line symbols.namesake Microscopy Electron ELECTRON TOMOGRAPHY symbols Cisternal lumen INTRACELLULAR-TRANSPORT Secretory pathway |
| Zdroj: | Nature cell biology 6 (2004). doi:10.1038/ncb1180 info:cnr-pdr/source/autori:Trucco A.; Polishchuk R.S.; Martella O.; Di Pentima A.; Fusella A.; Di Giandomenico D.; San Pietro E.; Beznoussenko G.V.; Polishchuk E.V.; Baldassarre M.; Buccione R.; Geerts W.J.;Koster A.J.; Burger K.N., Mironov A.A.; Luini A./titolo:Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments/doi:10.1038%2Fncb1180/rivista:Nature cell biology/anno:2004/pagina_da:/pagina_a:/intervallo_pagine:/volume:6 |
| ISSN: | 1465-7392 |
| DOI: | 10.1038/ncb1180 |
| Popis: | The organization of secretory traffic remains unclear, mainly because of the complex structure and dynamics of the secretory pathway. We have thus studied a simplified system, a single synchronized traffic wave crossing an individual Golgi stack, using electron tomography. Endoplasmic-reticulum-to-Golgi carriers join the stack by fusing with cis cisternae and induce the formation of intercisternal tubules, through which they redistribute their contents throughout the stack. These tubules seem to be pervious to Golgi enzymes, whereas Golgi vesicles are depleted of both enzymes and cargo. Cargo then traverses the stack without leaving the cisternal lumen. When cargo exits the stack, intercisternal connections disappear. These findings provide a new view of secretory traffic that includes dynamic intercompartment continuities as key players. |
| Databáze: | OpenAIRE |
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