Indole-3-Acetic Acid Produced by Burkholderia heleia Acts as a Phenylacetic Acid Antagonist to Disrupt Tropolone Biosynthesis in Burkholderia plantarii
| Autor: | Seiji Tachibana, Sharon Yu Ling Lau, Yuta Murai, Mengchao Cao, Guonian Zhu, Makoto Hashimoto, Li Li, Yasuyuki Hashidoko, Mengcen Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Multidisciplinary Metabolite 030106 microbiology Phenylalanine Phenylacetic acid Biology biology.organism_classification Tropolone Microbiology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry Biosynthesis Indole-3-acetic acid Bacteria Burkholderia plantarii |
| Zdroj: | Scientific Reports. |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep22596 |
| Popis: | Burkholderia heleia PAK1-2 is a potent biocontrol agent isolated from rice rhizosphere, as it prevents bacterial rice seedling blight disease caused by Burkholderia plantarii. Here, we isolated a non-antibacterial metabolite from the culture fluid of B. heleia PAK1-2 that was able to suppress B. plantarii virulence and subsequently identified as indole-3-acetic acid (IAA). IAA suppressed the production of tropolone in B. plantarii in a dose-dependent manner without any antibacterial and quorum quenching activity, suggesting that IAA inhibited steps of tropolone biosynthesis. Consistent with this, supplementing cultures of B. plantarii with either L-[ring-2H5]phenylalanine or [ring-2H2~5]phenylacetic acid revealed that phenylacetic acid (PAA), which is the dominant metabolite during the early growth stage, is a direct precursor of tropolone. Exposure of B. plantarii to IAA suppressed production of both PAA and tropolone. These data particularly showed that IAA produced by B. heleia PAK1-2 disrupts tropolone production during bioconversion of PAA to tropolone via the ring-rearrangement on the phenyl group of the precursor to attenuate the virulence of B. plantarii. B. heleia PAK1-2 is thus likely a microbial community coordinating bacterium in rhizosphere ecosystems, which never eliminates phytopathogens but only represses production of phytotoxins or bacteriocidal substances. |
| Databáze: | OpenAIRE |
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