Are cytokines and growth factors responsible for the detrimental effects of hydrosalpingeal fluid on pregnancy rates after in vitro fertilization-embryo transfer?
| Autor: | Steven D. Spandorfer, Larry I. Barmat, Ania Kowalik, el-Roeiy A, Kim Nasti, Xu Yang |
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| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Pregnancy Rate medicine.medical_treatment Fertilization in Vitro Biology Pregnancy Epidermal growth factor Internal medicine medicine Humans Growth Substances Hydrosalpinx Retrospective Studies In vitro fertilisation Growth factor Uterus Obstetrics and Gynecology Embryo Transfer medicine.disease Embryo transfer Body Fluids Endocrinology Cytokine medicine.anatomical_structure Reproductive Medicine Cytokines Female Tumor necrosis factor alpha Fallopian tube |
| Zdroj: | Fertility and Sterility. 72:1110-1112 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(99)00422-7 |
| Popis: | Objective: To characterize the secretion of cytokines and growth factors in hydrosalpingeal fluid. Design: Retrospective analysis. Setting: Hospital-based infertility practice. Patient(s): Ten infertile women who underwent laparoscopic aspiration of their hydrosalpingeal fluid before salpingectomy or neosalpingostomy. Intervention(s): Samples were cryopreserved, then thawed and centrifuged to remove cellular debris. Main Outcome Measure(s): The supernatants were analyzed for the presence of human interferon-γ, epidermal growth factor, transforming growth factor-β2, and tumor necrosis factor-α by quantitative enzyme immunoassay kits. Result(s): Interferon-γ and transforming growth factor-β2 were not detected in any of the hydrosalpingeal fluid samples. Epidermal growth factor was present in 5 of 10 hydrosalpingeal fluid samples, with a mean (±SE) concentration of 26.7 ± 11.4 pg/mL. Tumor necrosis factor-α was detected in 7 of 10 samples, with a mean (±SE) concentration of 6.2 ± 3.6 pg/mL. Three of the 10 samples contained both tumor necrosis factor-α and epidermal growth factor. Conclusion(s): For the first time, we described the absence of interferon-γ and transforming growth factor-β2, and the presence of epidermal growth factor and tumor necrosis factor-α in human hydrosalpingeal fluid. Because the fundamental role of the human fallopian tube is secretory in nature, the alteration in substances secreted from the tubal epithelium that reflux into the uterine cavity may explain the deleterious effects that hydrosalpingeal fluid has on pregnancy rates after IVF-ET. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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