Preparation, Single-Molecule Manipulation, and Energy Transfer Investigation of a Polyfluorene-graft -DNA polymer
| Autor: | Abhichart Krissanaprasit, Karina S Nielsen, Kurt V. Gothelf, Alexander N. Zelikin, Rasmus S. Christensen, Mette R Bakke, Camilla Frich Riber, Mikael Rask Madsen |
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| Rok vydání: | 2017 |
| Předmět: |
Polymers
DNA Single-Stranded Nanotechnology 02 engineering and technology Conjugated system Microscopy Atomic Force 010402 general chemistry 01 natural sciences Catalysis Polyfluorene chemistry.chemical_compound Fluorescence Resonance Energy Transfer DNA origami Molecule Nanoscopic scale chemistry.chemical_classification Fluorenes Quenching (fluorescence) Chemistry Organic Chemistry General Chemistry Polymer 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences Self-assembly 0210 nano-technology |
| Zdroj: | Madsen, M, Christensen, R S, Krissanaprasit, A, Bakke, M R, Riber, C F, Nielsen, K S, Zelikin, A N & Gothelf, K V 2017, ' Preparation, single-molecule manipulation and energy transfer investigation of a polyfluorene-graft-DNA polymer ', Chemistry: A European Journal, vol. 23, no. 44 . https://doi.org/10.1002/chem.201702780 |
| ISSN: | 0947-6539 |
| Popis: | Conjugated polymers have been intensively studied due to their unique optical and electronic properties combined with their physical flexibility and scalable bottom up synthesis. Although the bulk qualities of conjugated polymers have been extensively utilized in research and industry, the ability to handle and manipulate conjugated polymers at the nanoscale lacks significantly behind. Here, the toolbox for controlled manipulation of conjugated polymers was expanded through the synthesis of a polyfluorene-DNA graft-type polymer (poly(F-DNA)). The polymer possesses the characteristics associated with the conjugated polyfluorene backbone, but the protruding single-stranded DNA provides the material with an exceptional addressability. This study demonstrates controlled single-molecule patterning of poly(F-DNA), as well as energy transfer between two different polymer-DNA conjugates. Finally, highly efficient DNA-directed quenching of polyfluorene fluorescence was shown. |
| Databáze: | OpenAIRE |
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