Low-Affinity Nerve Growth Factor Receptor p75NTR Immunoreactivity in the Myocardium with Sympathetic Hyperinnervation

Autor:	B A Kai Chien, Che-Ming Chang, Hrayr S. Karagueuzian, B A Johnny Yi, Ji-Min Cao, Lan S. Chen, Ignacio Gonzalez-Gomez, Shengmei Zhou, Yasushi Miyauchi, Moshe Swissa, Peng-Sheng Chen, Kamlesh Asotra, Michael C. Fishbein, Katherine J. Fu
Rok vydání:	2004
Předmět:	medicine.medical_specialty Sympathetic Nervous System Sympathetic nerve Tropomyosin receptor kinase A Receptor Nerve Growth Factor Infusion Site Receptor tyrosine kinase Dogs Physiology (medical) Internal medicine Nerve Growth Factor Animals Low-affinity nerve growth factor receptor Medicine Receptor trkA Receptor Infusion Pumps NGF Receptor biology business.industry Myocardium Heart Control Groups Immunohistochemistry Death Sudden Cardiac Endocrinology Nerve growth factor nervous system biology.protein sense organs Cardiology and Cardiovascular Medicine business
Zdroj:	Journal of Cardiovascular Electrophysiology. 15:430-437
ISSN:	1540-8167 1045-3873
Popis:	Introduction: We previously demonstrated the relationship between sympathetic nerve density in myocardium and the occurrences of ventricular arrhythmia. Nerve growth factor (NGF) regulates myocardial sympathetic innervation. However, it is unclear whether the NGF high-affinity receptor tyrosine kinase A (TrkA) and the NGF low-affinity receptor p75NTR are altered in the state of sympathetic hyperinnervation in the heart. The aim of this study was to determine the density and location of TrkA and p75NTR in canine ventricles with sympathetic hyperinnervation. Methods and Results: Myocardial sympathetic hyperinnervation was induced by local infusion of NGF into myocardium or left stellate ganglia, or chronic subthreshold electric stimulation to the left stellate ganglia. The results showed that TrkA immunoreactivity was absent in the myocardium. Low-affinity receptor p75NTR immunoreactivity was present in axons, Schwann cells, and interstitial cells of sympathetic nerves, as well as in interstitial cells of the myocardium. The density of p75NTR immunolabeled myocardial interstitial cells at the NGF infusion site was lower than that at the site remote from NGF infusion, yet the sympathetic nerve density was higher at the infusion site than the remote area. The density of p75NTR also was lower in the myocardium with high sympathetic nerve density, induced by NGF infusion or chronic electric stimulation of the left stellate ganglia, compared to control groups. Conclusion: The data indicate that p75NTR may be the main NGF receptor in the myocardium, and p75NTR immunopositive interstitial cells may have a role in regulating sympathetic nerve growth in canine heart. (J Cardiovasc Electrophysiol, Vol. 15, pp. 430-437, April 2004)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5b23897e99c9e9f03032bccb46efbff https://doi.org/10.1046/j.1540-8167.2004.03517.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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