Toll-like receptor 2 and 4 ligation results in complex altered cytokine profiles early and late after burn injury
| Autor: | Bruce A. Cairns, Stefan Mlot, Anthony A. Meyer, C M. Barnes, Robert Maile |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Time Factors medicine.medical_treatment Peptidoglycan Critical Care and Intensive Care Medicine Sensitivity and Specificity Mice Random Allocation Immune system Reference Values medicine Animals Receptor Cells Cultured Toll-like receptor Innate immune system Cell Death business.industry Reverse Transcriptase Polymerase Chain Reaction Macrophages biochemical phenomena metabolism and nutrition Flow Cytometry Immunity Innate Toll-Like Receptor 2 Mice Inbred C57BL Toll-Like Receptor 4 TLR2 Disease Models Animal Cytokine Immunology TLR4 Cytokines Surgery Cytokine secretion Female Inflammation Mediators business Burns Spleen |
| Zdroj: | The Journal of trauma. 64(4) |
| ISSN: | 1529-8809 |
| Popis: | Toll-like receptors (TLR) 2 and TLR4 expressed on innate immune cells are important mediators of the immune response to pathogens. In this study, we hypothesized that burn injury results in altered cytokine secretion profiles after TLR2 or TLR4 ligation that is associated with altered TLR expression on innate immune cells.Female C56BL/6 mice were subjected to 20% full thickness burn or sham injury. Three or 14 days after injury whole splenocytes or purified splenic macrophages were cultured with TLR2 ligand peptidoglycan or TLR4 ligand lipopolysaccharide. Supernatants were assayed for TNF-alpha, MCP-1, IL-6 and IL-10. Cell death was assessed using flow cytometry. Innate CD11b F4/80 macrophages were sorted 14 days after burn injury and TLR2 and 4 expression was determined by quantitative reverse-transcriptase polymerase chain reaction and flow cytometry.Burn injury results in a steady accumulation in the periphery of CD11bF4/80 macrophages. Macrophages purified early after burn injury upregulated TLR2 and 4, followed by a decrease of TLR2 and TLR4 expression late after burn injury. TLR2 and TLR4 ligation of an equivalent number of purified macrophages 3 days after burn injury revealed no significant differences in cytokine secretion compared with sham. Stimulation 14 days after burn injury revealed a significant reduction in tumor necrosis factor-alpha secretion by macrophages compared with sham mice. In contrast, interleukin-10 was significantly increased (mean, approximately 1.8-fold) late after burn injury after either TLR2 or TLR4 stimulation. Interleukin-6 and monocyte chemotactic protein-1 secretion was unchanged from sham levels. In contrast, whole splenocyte stimulation resulted in increased cytokine 3 days and 14 days after burn injury. This effect is likely caused by the accumulation of TLR macrophages, which are resistant to TLR-induced cell death.Cytokine secretion profiles after TLR2 and TLR4 ligation after burn injury are altered in a manner not clearly reflective of an anti-inflammatory or proinflammatory state and are associated with unique changes in the macrophage population. TLR2 and TLR4 ligation have complex and varied roles in mediating the immune response to burn injury. |
| Databáze: | OpenAIRE |
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