Quantitative proteomics reveals the regulatory networks of circular RNA BTBD7_hsa_circ_0000563 in human coronary artery
| Autor: | Zhi-Yuan Mao, Chenhui Zhao, Yongjie Zhang, Qiao-Wei Jia, Lian-Sheng Wang, Mu-Feng Gu, Jianliang Jin, Jing Zhang, Jinxia Yuan, Haijian Sun, Lei Hua, Wen-Zhu Ma, En-Zhi Jia, Jiaxin Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics 0301 basic medicine Microbiology (medical) Atherosclerotic stenosis Proteome Clinical Biochemistry Quantitative proteomics Protein expression Coronary artery disease 03 medical and health sciences 0302 clinical medicine Circular RNA medicine Humans Immunology and Allergy circRNA Protein Interaction Maps coronary heart disease Binding site Research Articles Aged Chemistry Biochemistry (medical) Public Health Environmental and Occupational Health RNA Circular Hematology Middle Aged medicine.disease Coronary Vessels Molecular biology Coronary heart disease Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis BTBD7_hsa_circ_0000563 coronary artery segment Research Article Artery |
| Zdroj: | Journal of Clinical Laboratory Analysis |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/jcla.23495 |
| Popis: | Background BTBD7_hsa_circ_0000563, which is located on chromosome 14, contains conserved binding sites with miR‐155/130a and RNA‐binding proteins according to bioinformatic prediction. We investigated the association of BTBD7_hsa_circ_0000563 expression in coronary artery segments with atherosclerotic stenosis and identified the proteome‐wide BTBD7_hsa_circ_0000563‐regulated proteins in human coronary artery. Methods The atherosclerotic grade and extent in coronary artery segments were determined by hematoxylin and eosin staining. BTBD7_hsa_circ_0000563 expression in eight coronary artery segments from one patient was quantified by RT‐qPCR assay. A proteomic approach was adopted to reveal significant differences in protein expression between among four groups differing in their BTBD7_hsa_circ_0000563 expression levels. Results The RT‐qPCR assay revealed that coronary artery segments with severe atherosclerotic stenosis had significantly low BTBD7_hsa_circ_0000563 levels. The proteomic analysis identified 49 differentially expressed proteins among the segment groups with different BTBD7_hsa_circ_0000563 expression levels, of which 10 were downregulated and 39 were upregulated with increases in the BTBD7_hsa_circ_0000563 level. The 10 downregulated proteins were P61626 (LYSC_HUMAN), P02760 (AMBP_HUMAN), Q02985 (FHR3_HUMAN), P01701 (LV151_HUMAN), P06312(KV401_HUMAN), P01624 (KV315_HUMAN), P13671 (CO6_HUMAN), P01700(LV147_HUMAN), Q9Y287(ITM2B_HUMAN), and A0A075B6I0 (LV861_HUMAN). The top 10 upregulated proteins were Q92552 (RT27_HUMAN), Q9UJY1(HSPB8_HUMAN), Q9Y235(ABEC2_HUMAN), P19022 (CADH2_HUMAN), O43837(IDH3B_HUMAN), Q9H479(FN3K_HUMAN), Q9UM22(EPDR1_HUMAN), P48681(NEST_HUMAN), Q9NRP0(OSTC_HUMAN), and Q15628(TRADD_HUMAN). Conclusion BTBD7_hsa_circ_0000563 is involved in the atherosclerotic changes in human coronary artery segments. Verification, mechanistic, and function studies are needed to confirm whether patients with coronary artery disease would benefit from such personalized medicine in the future. We investigated BTBD7_hsa_circ_0000563 expression level in coronary artery segments with atherosclerotic stenosis and identified 49 differentially BTBD7_hsa_circ_0000563‐regulated proteins in human coronary artery which were mainly located in the mitochondria and involved in the citrate cycle (TCA cycle) pathway. It provided new insights into ATP synthase important role in the regulatory network of BTBD7_hsa_circ_0000563 in the coronary artery. |
| Databáze: | OpenAIRE |
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