Meroterpenoids from the Brown Alga Cystoseira usneoides as Potential Anti-Inflammatory and Lung Anticancer Agents
| Autor: | Miguel López-Lázaro, Hanaa Zbakh, José Manuel Calderón-Montaño, Eva Zubía, Virginia Motilva, Carolina de los Reyes |
|---|---|
| Přispěvatelé: | Química Orgánica, Universidad de Sevilla. Departamento de Farmacología, Junta de Andalucía |
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
meroterpenoids Pharmaceutical Science Pharmacology Anti-inflammatory Flow cytometry 03 medical and health sciences 0302 clinical medicine Drug Discovery medicine Cytotoxic T cell Cystoseira usneoides Cytotoxicity lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology A549 cell 0303 health sciences medicine.diagnostic_test Chemistry fungi Cell cycle cytokines lung cancer lcsh:Biology (General) inflammation 030220 oncology & carcinogenesis Cancer cell Tumor necrosis factor alpha cell cycle |
| Zdroj: | Mar. Drugs 2020, 18(4), 207 RODIN. Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz instname Marine Drugs, Vol 18, Iss 207, p 207 (2020) idUS. Depósito de Investigación de la Universidad de Sevilla Marine Drugs Volume 18 Issue 4 |
| Popis: | The anti-inflammatory and anticancer properties of eight meroterpenoids isolated from the brown seaweed Cystoseira usneoides have been evaluated. The algal meroterpenoids (AMTs) 1&ndash 8 were tested for their inhibitory effects on the production of the pro-inflammatory cytokines tumor necrosis factor (TNF-&alpha ), interleukin-6 (IL-6), and interleukin-1&beta (IL-1&beta ), and the expression of cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in LPS-stimulated THP-1 human macrophages. The anticancer effects were assessed by cytotoxicity assays against human lung adenocarcinoma A549 cells and normal lung fibroblastic MRC-5 cells, together with flow cytometry analysis of the effects of these AMTs on different phases of the cell cycle. The AMTs 1&ndash 8 significantly reduced the production of TNF-&alpha IL-6, and IL-1&beta and suppressed the COX-2 and iNOS expression, in LPS-stimulated cells (p < 0.05). The AMTs 1&ndash 8 displayed higher cytotoxic activities against A549 cancer cells than against MRC-5 normal lung cells. Cell cycle analyses indicated that most of the AMTs caused the arrest of A549 cells at the G2/M and S phases. The AMTs 2 and 5 stand out by combining significant anti-inflammatory and anticancer activities, while 3 and 4 showed interesting selective anticancer effects. These findings suggest that the AMTs produced by C. usneoides may have therapeutic potential in inflammatory diseases and lung cancer. |
| Databáze: | OpenAIRE |
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