Quantification of Protein Metabolismin Vivofor Skin, Wound, and Muscle in Severe Burn Patients
| Autor: | Dennis C. Gore, Steven E. Wolf, Robert R. Wolfe, David L. Chinkes, David N. Herndon, Arthur P. Sanford |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors 030309 nutrition & dietetics Phenylalanine Phenylalanine transport Protein metabolism Muscle Proteins Medicine (miscellaneous) Femoral artery Pharmacology 03 medical and health sciences chemistry.chemical_compound Injury Severity Score 0302 clinical medicine In vivo medicine.artery medicine Humans Muscle Skeletal Skin Leg Wound Healing 0303 health sciences Nutrition and Dietetics integumentary system business.industry Skeletal muscle Middle Aged Surgery Protein catabolism medicine.anatomical_structure chemistry Regional Blood Flow Wounds and Injuries Female 030211 gastroenterology & hepatology Basal Metabolism Burns business Blood sampling |
| Zdroj: | Journal of Parenteral and Enteral Nutrition. 30:331-338 |
| ISSN: | 1941-2444 0148-6071 |
| Popis: | In response to injury, muscle catabolism can be extensive, and in theory, the wound consumes amino acids to support healing. The purpose of this study is to assess a technique by which in vivo protein kinetics of muscle, wound, and normal skin can be quantified in burn-injured patients.Study protocol consisting of infusion of d5 phenylalanine; biopsies of skeletal muscle, skin, and donor-site wound on the leg; quantification of blood flow to total leg, wound, and skin; and sequential blood sampling from the femoral artery and vein. Five-compartment modeling was used to quantify the rates of protein synthesis, breakdown, and phenylalanine transport between muscle, wound, and skin.The study results demonstrated a net release of phenylalanine from muscle yet a net consumption of phenylalanine by the wound. Compared with skin, the wound had a substantially increased rate of protein synthesis and a reduced rate of protein breakdown (p.01). Transport rates into and out of muscle were significantly higher than those for wound (p.01).This novel methodology enables in vivo quantification of the integrated response of muscle, wound, and skin protein/amino acid metabolism and confirms the long-held theory of a net catabolism of muscle and a net anabolism of wound protein in patients after injury. This methodology can be used to assess the metabolic impact of such measures as nutrition, pharmacologic agents, and surgical procedures. |
| Databáze: | OpenAIRE |
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