DNA and origin region segregation are not affected by the transition from rod to sphere after inhibition of Escherichia coli MreB by A22
| Autor: | Aneta, Karczmarek, Rocío, Martínez-Arteaga, Rocío Martínez-Arteaga, Baselga, Svetlana, Alexeeva, Flemming G, Hansen, Miguel, Vicente, Nanne, Nanninga, Tanneke, den Blaauwen |
|---|---|
| Přispěvatelé: | Molecular Cytology (SILS, FNWI) |
| Rok vydání: | 2007 |
| Předmět: |
DNA Replication
DNA Bacterial Origin Recognition Complex Biology medicine.disease_cause Microbiology MreB Chromosome segregation chemistry.chemical_compound Chromosome Segregation medicine Escherichia coli Nucleoid Molecular Biology Actin Escherichia coli K12 Escherichia coli Proteins Chromosome Amdinocillin Gene Expression Regulation Bacterial Cell biology chemistry Cytoplasm DNA |
| Zdroj: | Molecular Microbiology, 65(1), 51-63. Wiley-Blackwell Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0950-382X |
| Popis: | Incluye 9 figuras y 1 tabla, más 2 figuras complementarias en archivo adjunto. The bacterial actin homologue MreB forms a helix underneath the cytoplasmic membrane and was shown to be essential in the morphogenesis of the rod-shaped cells. Additionally, MreB was implicated to be involved in DNA segregation. However, in our hands the mreBCD deletion strain (PA340-678) grew without apparent DNA segregation defect, suggesting that the reported chromosome segregation inhibition could be caused by a temporarily effect of MreB inhibition or depletion. To assess the involvement of MreB in DNA segregation during the transition from rod to sphere, we compared the effect of A22 and the PBP2 inhibitor mecillinam on the percentage of cells with segregated nucleoids and the number of oriC foci in wild-type Escherichia coli cells. Cells became spherical in the same time window during both treatments and we could not detect any difference in the chromosome or oriC segregation between these two treatments. Additionally, flow cytometric analyses showed that A22 and mecillinam treatment gave essentially the same chromosome segregation pattern. We conclude that MreB is not directly involved in DNA segregation of E. coli. This work was supported by a European FP6 STREP Grant ‘COBRA’ LSHM-CT-2003-503335 (A. Karczmarek), a Molecule to Cell Grant 805.47.200 (S. Alexeeva)and a Vernieuwingsimpuls Grant 016.001.024 (T. den Blaauwen) both of the Netherlands Organization for Scientific Research (N.W.O.). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text