Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis
| Autor: | Ya-Ping Li, Jia-Xin Li, Li-Min Xie, Xun-Jie Cao, Zi-Jian Leng, Xu-Guang Guo, Guo-Dong Zhu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Conjunctiva Chlamydia trachomatis Conjunctival Biology QH426-470 Proteomics medicine.disease_cause Reproductive Tract Infections 03 medical and health sciences 0302 clinical medicine Bioinformatics analysis Drug Discovery Gene expression medicine Genetics Humans Gene Regulatory Networks Protein Interaction Maps CD40 Antigens Molecular Biology Gene Fallopian Tubes MiRTarBase Chlamydia Computational Biology Granulocyte-Macrophage Colony-Stimulating Factor medicine.disease Human genetics MicroRNAs 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Host-Pathogen Interactions Molecular Medicine Medicine Female Primary Research Software Signal Transduction Reproductive tract |
| Zdroj: | Human Genomics Human Genomics, Vol 15, Iss 1, Pp 1-15 (2021) |
| ISSN: | 1479-7364 |
| Popis: | Background Currently, Chlamydia trachomatis–specific host defense mechanisms in humans remain poorly defined. To study the characteristics of host cells infected early with Chlamydia trachomatis, we used bioinformatics methods to analyze the RNA transcription profiles of the conjunctiva, fallopian tubes, and endometrium in humans infected with Chlamydia trachomatis. Method The gene expression profiles of GSE20430, GSE20436, GSE26692, and GSE41075 were downloaded from the Gene Expression Synthesis (GEO) database. Then, we obtained the differentially expressed genes (DEGs) through the R 4.0.1 software. STRING was used to construct protein–protein interaction (PPI) networks; then, the Cytoscape 3.7.2 software was used to visualize the PPI and screen hub genes. GraphPad Prism 8.0 software was used to verify the expression of the hub gene. In addition, the gene–miRNA interaction was constructed on the NetworkAnalyst 3.0 platform using the miRTarBase v8.0 database. Results A total of 600 and 135 DEGs were screened out in the conjunctival infection group and the reproductive tract infection group, respectively. After constructing a PPI network and verifying the hub genes, CSF2, CD40, and CSF3 in the reproductive tract infection group proved to have considerable statistical significance. Conclusion In our research, the key genes in the biological process of reproductive tract infection with Chlamydia trachomatis were clarified through bioinformatics analysis. These hub genes may be further used in clinical treatment and clinical diagnosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink