Structural and Biophysical Characterization of the HCV E1E2 Heterodimer for Vaccine Development
| Autor: | Andrezza Chagas, Eric A. Toth, Brian G. Pierce, Thomas R. Fuerst |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
biophysical characterization Models Molecular Viral Hepatitis Vaccines E1E2 glycoprotein complex Protein Conformation Hepatitis C virus Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Computational biology Review Hepacivirus Biology Vaccine antigen medicine.disease_cause Microbiology Epitope E1 03 medical and health sciences Epitopes Mice Immune system Antigen E2 Viral Envelope Proteins Glycoprotein complex Virology protein purification medicine Animals Humans secreted E1E2 protein expression 030102 biochemistry & molecular biology envelope glycoproteins Hepatitis C QR1-502 Functional integrity 030104 developmental biology Infectious Diseases HEK293 Cells vaccine design Protein Multimerization hepatitis C virus (HCV) |
| Zdroj: | Viruses Viruses, Vol 13, Iss 1027, p 1027 (2021) |
| ISSN: | 1999-4915 |
| Popis: | An effective vaccine for the hepatitis C virus (HCV) is a major unmet medical and public health need, and it requires an antigen that elicits immune responses to multiple key conserved epitopes. Decades of research have generated a number of vaccine candidates; based on these data and research through clinical development, a vaccine antigen based on the E1E2 glycoprotein complex appears to be the best choice. One bottleneck in the development of an E1E2-based vaccine is that the antigen is challenging to produce in large quantities and at high levels of purity and antigenic/functional integrity. This review describes the production and characterization of E1E2-based vaccine antigens, both membrane-associated and a novel secreted form of E1E2, with a particular emphasis on the major challenges facing the field and how those challenges can be addressed. |
| Databáze: | OpenAIRE |
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