Influence of the complex drug Cocarnit on the sciatic nerve in the development of diabetic polyneuropathy in rats
| Autor: | Tetyana Beregova, Serhii Berehoviy, Olena G. Kuryk, Nataliia Nikitina, Ludmila I. Stepanova, Olexiy Savchuk, Ludmila Ostapchenko |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Drug ngf media_common.quotation_subject Pharmacology toxicology Pharmacy Biochemistry 03 medical and health sciences diabetic polyneuropathy 0302 clinical medicine Diabetic polyneuropathy Medicine Molecular Biology vegf media_common Pharmacology business.industry General Medicine bfgf active proteolytic enzymes cocarnit 030104 developmental biology Anesthesia Sciatic nerve business 030217 neurology & neurosurgery |
| Zdroj: | Current Issues in Pharmacy and Medical Sciences, Vol 33, Iss 3, Pp 113-120 (2020) |
| ISSN: | 2300-6676 2084-980X |
| Popis: | Ulcers and slow wound healing are common in diabetic polyneuropathy (DP), as well as shooting or burning pain, sensitivity to touch or lack of sensitivity, low oxygenation of nerve tissue, conductivity disorders and various vascular disorders. The mechanisms of DP development are complex and have not been completely studied. To take into account the role of B group vitamins, we investigated histological structure of nerve tissue, the level of different growth factors and the qualitative composition of active proteolytic enzymes in rats with DP and after the use of the metabolic drug Cocarnit for 9 days. This drug composition include nicotinamide, cocarboxylase, cyanocobalamin, adenosine triphosphate disodium trihydrate. We used an histological study of sciatic nerve; enzyme-linked immunosorbent assay and enzyme electrophoresis methods. In rats with DP, fragmentation of nerve tissue and their necrosis was established. Moreover, degraded forms of plasmin that has a fully functional serine proteinase domain are evident, and, therefore, it exhibits proteolytic properties. DP led to a decrease of neuron growth factor (NGF), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). After treatment, the histological structure of nerve tissue was significantly improved, and the expression of growth factors NGF and bFGF was increased. Our study demonstrated that administration of Corcarnit brought about the complete restoration of the activation potential of plasmin and the almost disappearance of all degraded forms which were evident in the group with DP. |
| Databáze: | OpenAIRE |
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