Water dependent structural changes of silk from Bombyx mori gland to fibre as evidenced by Raman and IR spectroscopies
| Autor: | Hung Manh Dinh, Philippe Colomban, Aline Percot, Céline Paris, Marine Wojcieszak, Bernard Mauchamp |
|---|---|
| Přispěvatelé: | Université Pierre et Marie Curie - Paris 6 (UPMC), De la Molécule aux Nanos-objets : Réactivité, Interactions et Spectroscopies (MONARIS), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Gland
[SDV]Life Sciences [q-bio] Silk Infrared spectroscopy Fibroin Fibre chemistry.chemical_compound symbols.namesake Bombyx mori Side chain Spider silk Carboxylate Conformation Raman Spectroscopy biology Chemistry fungi technology industry and agriculture Water biology.organism_classification Crystallography SILK Biochemistry symbols Raman spectroscopy ATR-FTIR |
| Zdroj: | Vibrational Spectroscopy Vibrational Spectroscopy, Elsevier, 2014, 73, pp.79-89. ⟨10.1016/j.vibspec.2014.05.004⟩ Vibrational Spectroscopy, 2014, 73, pp.79-89. ⟨10.1016/j.vibspec.2014.05.004⟩ |
| ISSN: | 0924-2031 |
| DOI: | 10.1016/j.vibspec.2014.05.004⟩ |
| Popis: | International audience; DSC, attenuated total reflexion infrared (ATR-FTIR), and Raman microspectroscopy were used for the first time in a close to in vivo environment to study ready-to-spin Bombyx mori silkworms. The aim was to understand the change of the fibroin backbone organisation from the gland to the fibre. Raman shifts of the Amide I components reveal a strong change of organisation in the middle part of the hydrated gland, as anticipated previously measured modifications of salts concentrations and pH. Series of bands characteristics of the fully hydrated silk disappear, as observed for spider silk, despite the different aminoacid sequence. Confirmation is obtained from IR spectra taking into account the superimposed water component. The change of the silk water interaction in the central part of the gland, from a hydrophobic to hydrophilic behaviour, is related to the water content decrease along the gland. pH sensitive carboxylate side chains markers confirm the modification. Fibroin organisation was also studied in the dried gland and in the spun fibre. The fibre extrusion by orients the fibroin chains along the fibre axis, with intercalated water molecules, leading to a material with specific mechanical properties, compared to the amorphous dried gland. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect