Apoptotic Effects on HL60 Human Leukaemia Cells Induced by Lavandin Essential Oil Treatment

Autor: Elisa Ovidi, Giovanni Turchetti, Stefania Garzoli, Pierluigi Giacomello, Valentina Laghezza Masci, Antonio Tiezzi, Anna Rita Taddei
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Programmed cell death
caspase-3
apoptosis
electron microscopy
flow cytometry
HL60
Immunofluorescence techniques
lavandin essential oil
MTT test
Cell Survival
hl60
Pharmaceutical Science
Fluorescent Antibody Technique
HL-60 Cells
Linalyl acetate
Article
Analytical Chemistry
Flow cytometry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
lcsh:Organic chemistry
Annexin
Drug Discovery
medicine
Oils
Volatile
Humans
Plant Oils
Viability assay
Physical and Theoretical Chemistry
030304 developmental biology
immunofluorescence techniques
0303 health sciences
mtt test
medicine.diagnostic_test
Chemistry
Caspase 3
Organic Chemistry
Molecular biology
Lavandula
Chemistry (miscellaneous)
Apoptosis
030220 oncology & carcinogenesis
Cancer cell
Molecular Medicine
Zdroj: Molecules, Vol 25, Iss 3, p 538 (2020)
Molecules
Volume 25
Issue 3
ISSN: 1420-3049
Popis: Recent scientific investigations have reported a number of essential oils to interfere with intracellular signalling pathways and to induce apoptosis in different cancer cell types. In this paper, Lavandin Essential Oil (LEO), a natural sterile hybrid obtained by cross-breeding L. angustifolia&thinsp
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&thinsp
L. latifolia, was tested on human leukaemia cells (HL60). Based on the MTT results, the reduced cell viability of HL60 cells was further investigated to determine whether cell death was related to the apoptotic process. HL60 cells treated for 24 h with LEO were processed by flow cytometry, and the presence of Annexin V was measured. The activation of caspases-3 was evaluated by western blot and immunofluorescence techniques. Treated cells were also examined by scanning and transmission electron microscopy to establish the possible occurrence of morphological alterations during the apoptotic process. LEO main compounds, such as linalool, linalyl acetate, 1,8-cineole, and terpinen-4-ol, were also investigated by MTT and flow cytometry analysis. The set of obtained results showed that LEO treatments induced apoptosis in a dose-dependent, but not time-dependent, manner on HL60 cells, while among LEO main compounds, both terpinen-4-ol and linalyl acetate were able to induce apoptosis.
Databáze: OpenAIRE
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