Glutamatergic Signaling by Mesolimbic Dopamine Neurons in the Nucleus Accumbens
| Autor: | Fatuel Tecuapetla, Harry S. Xenias, Tibor Koós, Margaret E. Rice, Fulva Shah, James M. Tepper, Jyoti C. Patel, Karl Deisseroth, Daniel F. English, Ibrahim Tadros, Joshua Berlin |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
endocrine system Patch-Clamp Techniques Dopamine Population Dopamine Agents Glutamic Acid Tetrodotoxin Nucleus accumbens Neurotransmission Optogenetics Biology In Vitro Techniques Synaptic Transmission Nucleus Accumbens Glutamatergic Mice Postsynaptic potential Electrochemistry Animals education Neurons education.field_of_study General Neuroscience Dopaminergic Gene Transfer Techniques Excitatory Postsynaptic Potentials Electric Stimulation Luminescent Proteins nervous system Excitatory postsynaptic potential Female Brief Communications Neuroscience Photic Stimulation Signal Transduction Sodium Channel Blockers |
| Popis: | Recent evidence suggests the intriguing possibility that midbrain dopaminergic (DAergic) neurons may use fast glutamatergic transmission to communicate with their postsynaptic targets. Because of technical limitations, direct demonstration of the existence of this signaling mechanism has been limited to experiments using cell culture preparations that often alter neuronal function including neurotransmitter phenotype. Consequently, it remains uncertain whether glutamatergic signaling between DAergic neurons and their postsynaptic targets exists under physiological conditions. Here, using an optogenetic approach, we provide the first conclusive demonstration that mesolimbic DAergic neurons in mice release glutamate and elicit excitatory postsynaptic responses in projection neurons of the nucleus accumbens. In addition, we describe the properties of the postsynaptic glutamatergic responses of these neurons during experimentally evoked burst firing of DAergic axons that reproduce the reward-related phasic population activity of the mesolimbic projection. These observations indicate that, in addition to DAergic mechanisms, mesolimbic reward signaling may involve glutamatergic transmission. |
| Databáze: | OpenAIRE |
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