Viral vector-mediated expression of K+ channels regulates electrical excitability in skeletal muscle
| Autor: | Andrea J. Yool, Scott J. Sherman, R. K. Kilani, Torsten Falk |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Patch-Clamp Techniques Potassium Channels viruses Genetic Vectors Green Fluorescent Proteins Hypokalemic Periodic Paralysis Action Potentials Gene Expression Biology Transfection Recombinant virus Viral vector Green fluorescent protein Rats Sprague-Dawley Cnidarian Venoms Internal medicine Genetics medicine Animals Simplexvirus Hyperkalemic periodic paralysis Muscle Skeletal Molecular Biology Cells Cultured K channels Skeletal muscle Periodic paralysis Genetic Therapy medicine.disease Rats Cell biology Luminescent Proteins Endocrinology medicine.anatomical_structure Herpesvirus hominis Potassium Channels Voltage-Gated Kv1.4 Potassium Channel Molecular Medicine |
| Zdroj: | Gene Therapy. 8:1372-1379 |
| ISSN: | 1476-5462 0969-7128 |
| DOI: | 10.1038/sj.gt.3301539 |
| Popis: | Modification of K+ currents by exogenous gene expression may lead to therapeutic interventions in skeletal muscle diseases characterized by alterations in electrical excitability. In order to study the specific effects of increasing outward K+ currents, we expressed a modified voltage-dependent K+ channel in primary cultured rat skeletal muscle cells. The rat Kv1.4 channel was expressed as an N-terminal fusion protein containing a bioluminescent marker (green fluorescent protein). Transgene expression was carried out using the helper-dependent herpes simplex 1 amplicon system. Transduced myoballs, identified using fluorescein optics and studied electrophysiologically with single-cell patch clamp, exhibited a greater than two-fold increase in K+ conductance by 20-30 h after infection. This increase in K+ current led to a decrease in membrane resistance and a 10-fold increase in the current threshold for action potential generation. Electrical hyperexcitability induced by the Na+ channel toxin anemone toxin II (1 microM) was effectively counteracted by overexpression of Kv1.4 at 30-32 h after transduction. Thus, virally induced overexpression of a voltage-gated K+ channel in skeletal muscle has a powerful effect in reducing electrical excitability. |
| Databáze: | OpenAIRE |
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