ML0405 and ML2331 Are Antigens of Mycobacterium leprae with Potential for Diagnosis of Leprosy
| Autor: | Patrick J. Brennan, John S. Spencer, Greg C. Ireton, Nathan A. Groathouse, Robert H. Gelber, Wakako Goto, Raodoh Mohamath, Steven G. Reed, Jeffrey A. Guderian, Stephen T. Reece |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Clinical Biochemistry Immunology Immunoglobulin G Serology Antigen-Antibody Reactions Glycolipid Antigen Bacterial Proteins Sequence Analysis Protein Leprosy medicine Immunology and Allergy Humans Mycobacterium leprae Lepromatous leprosy Antigens Bacterial biology medicine.disease biology.organism_classification Antibodies Bacterial Leprosy Tuberculoid Leprosy Lepromatous biology.protein Microbial Immunology Antibody Glycolipids |
| Popis: | Despite the success of multidrug therapy in reducing the number of registered leprosy cases worldwide, evidence suggests thatMycobacterium lepraecontinues to be transmitted. A serological diagnostic test capable of identifying and allowing treatment of early-stage disease could reduce transmission and prevent the onset of the disability, a common complication of the disease in later stages. Serological diagnosis based on antibody recognition of phenolic glycolipid I (PGL-I) cannot reliably identify individuals with lower bacterial indices (BI). One strategy that might improve this situation is the provision of highly specific serological antigens that may be combined with PGL-I to improve the sensitivity of diagnosis. Using serological expression cloning with a serum pool of untreated lepromatous leprosy (LL) patients, we identified 14 strongly reactiveM. lepraeproteins, 5 of which were previously unstudied. We present results suggesting that two of these proteins, ML0405 and ML2331, demonstrate the ability to specifically identify LL/borderline lepromatous (BL) patients on the basis of immunoglobulin G (IgG) reactivity. In a household contact study, LL index cases were identified on the basis of this reactivity, while household contacts of these patients demonstrated undetectable reactivity. At a serum dilution of 1:800, suitable to reduce background PGL-I IgM reactivity, two BL patients with a BI of |
| Databáze: | OpenAIRE |
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