Overproduction of the Escherichia coli Chaperones GroEL-GroES in Rhodococcus ruber Improves the Activity and Stability of Cell Catalysts Harboring a Nitrile Hydratase
| Autor: | Hui Min Yu, Yu Xuan Tian, Jie Chen, Zhong Yao Shen |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Folding Biology medicine.disease_cause Applied Microbiology and Biotechnology Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound Nitrile hydratase Heat shock protein Enzyme Stability Chaperonin 10 Escherichia coli medicine Rhodococcus Overproduction Heat-Shock Proteins Hydro-Lyases Acrylamide Acrylonitrile Escherichia coli Proteins General Medicine GroES biology.organism_classification GroEL Recombinant Proteins 030104 developmental biology chemistry Biochemistry Biocatalysis Molecular Chaperones Plasmids Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 26:337-346 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.1509.09084 |
| Popis: | Three combinations of molecular chaperones from Escherichia coli (i.e., DnaK-DnaJ-GrpEGroEL- GroES, GroEL-GroES, and DnaK-DnaJ-GrpE) were overproduced in E. coli BL21, and their in vitro stabilizing effects on a nitrile hydratase (NHase) were assessed. The optimal gene combination, E. coli groEL-groES (ecgroEL-ES), was introduced into Rhodococcus ruber TH3. A novel engineered strain, R. ruber TH3G was constructed with the native NHase gene on its chromosome and the heterologous ecgroEL-ES genes in a shuttle plasmid. In R. ruber TH3G, NHase activity was enhanced 37.3% compared with the control, TH3. The in vivo stabilizing effect of ecGroEL-ES on the NHase was assessed using both acrylamide immersion and heat shock experiments. The inactivation behavior of the in vivo NHase after immersion in a solution of dynamically increased concentrations of acrylamide was particularly evident. When the acrylamide concentration was increased to 500 g/l (50%), the remaining NHase activity in TH3G was 38%, but in TH3, activity was reduced to 10%. Reactivation of the in vivo NHases after varying degrees of inactivation was further assessed. The activity of the reactivated NHase was more than 2-fold greater in TH3G than in TH3. The hydration synthesis of acrylamide catalyzed by the in vivo NHase was performed with continuous acrylonitrile feeding. The final concentration of acrylamide was 640 g/l when catalyzed by TH3G, compared with 490 g/l acrylamide by TH3. This study is the first to show that the chaperones ecGroEL-ES work well in Rhodococcus and simultaneously possess protein-folding assistance functions and the ability to stabilize and reactivate the native NHases. |
| Databáze: | OpenAIRE |
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