Double Coupling Edman Chemistry for High-Sensitivity Automated Protein Sequencing
| Autor: | Norman J. Dovichi, Annika Renborg, Xing-Fang Li, Darren F. Lewis, Ian D. Ireland, Min Chen, Sarah Kwong |
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| Rok vydání: | 1997 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography Chemistry Proteins Sensitivity and Specificity Biochemistry Fluorescence Amino acid Low volume Protein sequencing Capillary electrophoresis Phenylthiohydantoin Amino Acid Sequence Amino Acids Sequence Analysis Fluorescein-5-isothiocyanate |
| Zdroj: | Journal of Protein Chemistry. 16:491-493 |
| ISSN: | 1573-4943 0277-8033 |
| Popis: | Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a promising new method for the analysis of protein sequencing products. It gives 10 zmol (1 zmol = 10(-21) mol) limits of detection (3 sigma) for fluorescein thiohydantoin (FTH) amino acids. We have developed a separation for the (FTH)-amino acid products generated from 18 of the 20 coded amino acids. The extremely low volume requirement associated with CE-LIF makes it incompatible with commercial sequencers. For this reason, we have also been developing a miniaturized sequencer that can be more easily coupled to our detection system. Both the CE-LIF system and the miniaturized sequencer are described. |
| Databáze: | OpenAIRE |
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