Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID‐19
| Autor: | Rong Deng, Yunfei An, Bei Cai, Yi Li, Lanlan Wang, Xue-Dan Gao, Lin Yan, Li-Chun Wang, Huan Xu, Dongdong Li, Min-Jin Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male 0301 basic medicine China NK T Follicular Helper Cells CD226 medicine.medical_treatment Lymphocyte TIM‐3 Tfh CD8-Positive T-Lymphocytes Antibodies Viral Proinflammatory cytokine Young Adult 03 medical and health sciences 0302 clinical medicine Immune system COVID‐19 medicine Humans Aged Innate immune system biology SARS-CoV-2 business.industry COVID-19 Original Articles CD8 Cell Biology Middle Aged Killer Cells Natural 030104 developmental biology Cytokine medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology biology.protein Cytokines Molecular Medicine Original Article Female Antibody business |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.16044 |
| Popis: | Recent studies have demonstrated a marked decrease in peripheral lymphocyte levels in patients with coronavirus disease 2019 (COVID‐19) caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Few studies have focused on the changes of NK, T‐ and B‐cell subsets, inflammatory cytokines and virus‐specific antibodies in patients with moderate COVID‐19. A total of 11 RT‐PCR‐confirmed convalescent patients with COVID‐19 and 11 patients with non‐SARS‐CoV‐2 pneumonia (control patients) were enrolled in this study. NK, CD8+ T, CD4+ T, Tfh‐like and B‐cell subsets were analysed using flow cytometry. Cytokines and SARS‐CoV‐2‐specific antibodies were analysed using an electrochemiluminescence immunoassay. NK cell counts were significantly higher in patients with COVID‐19 than in control patients (P = 0.017). Effector memory CD8+ T‐cell counts significantly increased in patients with COVID‐19 during a convalescent period of 1 week (P = 0.041). TIM‐3+ Tfh‐like cell and CD226+ Tfh‐like cell counts significantly increased (P = 0.027) and decreased (P = 0.022), respectively, during the same period. Moreover, ICOS+ Tfh‐like cell counts tended to decrease (P = 0.074). No abnormal increase in cytokine levels was observed. The high expression of NK cells is important in innate immune response against SARS‐CoV‐2. The increase in effector memory CD8+ T‐cell counts, the up‐regulation of inhibitory molecules and the down‐regulation of active molecules on CD4+ T cells and Tfh‐like cells in patients with COVID‐19 would benefit the maintenance of balanced cellular and humoural immune responses, may prevent the development of severe cases and contribute to the recovery of patients with COVID‐19. |
| Databáze: | OpenAIRE |
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