A note on a simple estimation of amphetamine, methylamphetamine and ephedrine in horse urine
| Autor: | S. K. Wahba, A. R. Kozman, M. A. El‐Keiy, Mohamed S Karawya |
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| Rok vydání: | 1968 |
| Předmět: |
Ephedrine
Pharmacology chemistry.chemical_classification Chromatography Chemistry Silica gel Elution Sodium Acetaldehyde Pharmaceutical Science chemistry.chemical_element Urine Methamphetamine Amphetamine chemistry.chemical_compound Spectrophotometry Triethanolamine Methods medicine Animals Chromatography Thin Layer Horses Dithiocarbamate medicine.drug |
| Zdroj: | Journal of Pharmacy and Pharmacology. 20:650-652 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1111/j.2042-7158.1968.tb09828.x |
| Popis: | A chromatographic separation of amphetamine, methylamphetamine and ephedrine from horse urine is possible on alkaline Silica Gel G plates developed with acetone-methanol (1:3). After elution, the bases are determined colorimetrically. The intensity of the violet colour resulting from the nitration of amphetamine is measured in a Unicam SP1300 colorimeter using filter No. 1 (sensitivity 50–250 μg). The colour produced by the interaction of methylamphetamine, sodium nitro-prusside, acetaldehyde and triethanolamine is measured at 590 mμ (sensitivity 200–2,000 μg). Ephedrine was determined by measuring the intensity of the brown colour of its dithiocarbamate at 440 mμ (sensitivity 100–900 μg). |
| Databáze: | OpenAIRE |
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