Expression heterogeneity research of ITGB3 and BCL-2 in lung adenocarcinoma tissue and adenocarcinoma cell line
| Autor: | Zong-jiang Xia, Guo-Ju Sun, Yue-bin Wang, Kun Zhou, Wei Hu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lung adenocarcinoma
Oncology medicine.medical_specialty Lung Neoplasms Integrin Adenocarcinoma of Lung Biology Adenocarcinoma Transfection Metastasis Internal medicine Cell Line Tumor medicine Humans Lung cancer Lung A549 cell Medicine(all) Tissue microarray Integrin beta3 General Medicine medicine.disease B cell lymphoma Lymphoma medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Tissue Array Analysis Cancer research Immunohistochemistry Heterogeneity |
| Zdroj: | Asian Pacific Journal of Tropical Medicine. 7(6):473-477 |
| ISSN: | 1995-7645 |
| DOI: | 10.1016/s1995-7645(14)60077-8 |
| Popis: | ObjectiveTo analyze expression heterogeneity of Integrin beta 3 (ITGB3) and B-cell lymphoma 2 (BCL-2) in lung adenocarcinoma tissue and adenocarcinoma cell line and further provide theoretical direction for molecular biological research of lung adenocarcinoma.MethodsTissue microarray was used to observe relation among expression, heterogeneitpy and clinical characteristics of ITGB3 and BCL-2 in lung cancer.ResultsITGB3 and BCL-2 increased significantly in A549 cells in CAFs group withβ-actin as control; the expression level of BCL-2 also increased in ITGB3 transfected cells with GFP plasmid transfected A549 cells as control; immunohistochemistry staining showed that positive rates of ITGB3, ITGB1 and BCL-2 in normal lung tissues were 0, the positive rates in lung adenocarcinoma were 7.04%, 84.51% and 4.23%, respectively; in the results of immunohistochemistry staining, the expression of Girdin protein in lung adenocarcinoma was homogeneous, however protein expression of ITGB3, ITGB1 and BCL-2 showed different patterns in the same location with significant heterogeneity; majority of ITGB3, ITGB1 or BCL-2 positive tissue showed heterogeneity that expression in trailing edge was higher than that of trailing edge in lung adenocarcinoma tissue, the patients with BCL-2 heterogeneity showed higher lymph node metastasis ratio and lower clinical stage (P0.05).ConclusionsExpression of ITGB3 and BCL-2 in lung adenocarcinoma and adenocarcinoma cell line showed heterogeneity that expression in trailing edge was higher than that of trailing edge, which may play an important role in promoting tumor lymph node metastasis and vascular invasion, and provides a new research direction for exploration of lung adenocarcinoma metastasis mechanism. |
| Databáze: | OpenAIRE |
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