Local inflammation is associated with aortic thrombus formation in abdominal aortic aneurysms
| Autor: | Karol Urbanski, Tomasz Mikolajczyk, Agnieszka Sagan, Ryszard Nosalski, Mateusz Siedlinski, Tomasz J. Guzik, Ryszard Korbut, Wojciech Mrowiecki |
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| Rok vydání: | 2012 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Receptors CCR5 Lymphocyte T cell Lymphocytes Null 030204 cardiovascular system & hematology Lymphocyte Activation Article Monocytes 03 medical and health sciences 0302 clinical medicine Risk Factors T-Lymphocyte Subsets Leukocytes medicine Humans cardiovascular diseases IL-2 receptor Platelet activation Thrombus Aged Inflammation Aortitis business.industry Monocyte Thrombosis Hematology Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure cardiovascular system Female business CD8 circulatory and respiratory physiology Aortic Aneurysm Abdominal Blood vessel |
| Zdroj: | Thrombosis and Haemostasis. 108:812-823 |
| ISSN: | 2567-689X 0340-6245 |
| DOI: | 10.1160/th12-05-0339 |
| Popis: | SummaryIntraluminal thrombus formation in aortic abdominal aneurysms (AAA) is associated with adverse clinical prognosis. Interplay between coagulation and inflammation, characterised by leukocyte infiltration and cytokine production, has been implicated in AAA thrombus formation. We studied leukocyte (CD45+) content by flow cytometry in AAA thrombi from 27 patients undergoing surgical repair. Luminal parts of thrombi were leukocyte-rich, while abluminal segments showed low leukocyte content. CD66b+ granulocytes were the most prevalent, but their content was similar to blood. Monocytes (CD14+) and T cells (CD3+) were also abundant, while content of B lymphocytes (CD19+) and NK cells (CD56+CD16+) were low. Thrombi showed comparable content of CD14highCD16− monocytes and lower CD14highCD16+ and CD14dimCD16+, than blood. Monocytes were activated with high CD11b, CD11c and HLA-DR expression. Total T cell content was decreased in AAA thrombus compared to peripheral blood but CD8 and CD3+CD4-CD8− (double negative T cell) contents were increased in thrombi. CD4+ cells were lower but highly activated (high CD69, CD25 and HLA-DR). No differences in T regulatory (CD4+CD25+FoxP3+) cell or pro-atherogenic CD4+CD28null lymphocyte content were observed between thrombi and blood. Thrombus T cells expressed high levels of CCR5 receptor for chemokine RANTES, commonly released from activated platelets. Leukocyte or T cell content in thrombi was not correlated with aneurysm size. However, CD3+ content was significantly associated with smoking in multivariate analysis taking into account major risk factors for atherosclerosis. In conclusion, intraluminal AAA thrombi are highly inflamed, predominantly with granulocytes, CD14highCD16− monocytes and activated T lymphocytes. Smoking is associated with T cell infiltration in AAA intraluminal thrombi. |
| Databáze: | OpenAIRE |
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