Decreased mRNA Expressions of CD40L in Patients with Neuromyelitis Optica Spectrum Disorder
| Autor: | Ju Liu, Qin Zhang, Hongxi Chen, Qin Du, Hongyu Zhou, Zhiyun Lian, Mu Yang, Ying Zhang, Ling Feng, Ziyan Shi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Neurology CD40 Ligand Peripheral blood mononuclear cell Monocytes Pathogenesis 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Humans Neurochemistry RNA Messenger Autoimmune disease Messenger RNA Neuromyelitis optica CD40 biology business.industry Neuromyelitis Optica hemic and immune systems General Medicine Middle Aged medicine.disease 030104 developmental biology Immunology biology.protein Female business 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Journal of molecular neuroscience : MN. 70(4) |
| ISSN: | 1559-1166 |
| Popis: | Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune disease that preferentially affects central nerve system. Herein, we evaluated changes of CD40L and CD40 mRNA expressions in NMOSD and controls to explore their potential roles in development of NMOSD. The expressions of CD40L and CD40 mRNA in peripheral blood mononuclear cells (PBMCs) from patients with NMOSD and healthy controls were detected by quantitative real-time PCR (qPCR). Kruskal-Wallis tests were used to compare expression levels of CD40L and CD40 mRNA between groups, and Spearman correlation analysis was performed to evaluate correlation between mRNA expression levels and annual relapse rate (ARR) of NMOSD. A total of 71 patients with NMOSD and 42 gender- and age-matched healthy volunteers were recruited in our study. Compared with healthy controls, expression of CD40L mRNA was significantly decreased in untreated patients with NMOSD, and similar trends were observed also in CD40 mRNA expression although the difference was not significant. Other than that, immunosuppressants not only successfully increased CD40L and CD40 mRNA levels during remission of NMOSD, but also corrected the negative correlation between CD40L mRNA expression and annual relapse rate (ARR) of patients NMOSD. These results favored the long-term prognosis of NMOSD patients. Our results suggest that decreased expressions of CD40L mRNA may be involved in developing of NMOSD and the proper CD40L mRNA levels benefit to prevent attacks of NMOSD. Nevertheless, the relationship between protein and mRNA expressions of CD40L and their underlying roles in the pathogenesis of NMOSD remains to be further studied. |
| Databáze: | OpenAIRE |
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