Captopril apparently increase and cisplatin apparently decrease human albumin concentration in artificial urinary solutions
| Autor: | Rafał Kuzioła, Barbara Marczewska, Krzysztof Marczewski |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty Captopril Urinary system Clinical Biochemistry Serum Albumin Human Urine Urinalysis Models Biological 03 medical and health sciences Internal medicine medicine Immunology and Allergy Humans Fourier transform infrared spectroscopy Research Articles Cisplatin Kidney Chromatography Chemistry Biochemistry (medical) Public Health Environmental and Occupational Health Albumin Native Polyacrylamide Gel Electrophoresis Hematology body regions Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Endocrinology embryonic structures medicine.drug |
| Zdroj: | Journal of clinical laboratory analysis. 31(6) |
| ISSN: | 1098-2825 |
| Popis: | Background Measurement of urinary albumin (HSA) is very important in diagnostic of kidney diseases. Much less is known about the possible impact of substances present in urine together with albumin on the results of measurements. Methods We investigated the effect of the presence of captopril and cisplatin in the solution on the result of the determination of HSA by native polyacrylamide gel electrophoresis. Protein conformation in the absence and presence of the drugs was examined using Fourier Transform Infrared Spectroscopy (FTIR). Results The presence of captopril apparently increases HSA concentration while cisplatin causes an apparent decrease in the HSA concentration. The presence of both drugs also influence the secondary structure forms of HSA albumin investigated by FTIR. Conclusion Both drugs tested in the concentration of human use can have an impact on the results of determination of albumin in urine which can influence clinical decision. |
| Databáze: | OpenAIRE |
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