Cardiovascular Tissue Engineering: A New Laminar Flow Chamber for In Vitro Improvement of Mechanical Tissue Properties
| Autor: | Stefan Jockenhoevel, Simon P. Hoerstrup, Gregor Zünd, Marco Turina, AM Schnell |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Bicarbonate
Biomedical Engineering Biophysics Peristaltic pump Bioengineering Biomaterials Extracellular matrix Hydroxyproline chemistry.chemical_compound Tissue engineering Humans Aorta Cells Cultured HEPES Tissue Engineering Laminar flow General Medicine Carbon Dioxide Fibroblasts Culture Media Extracellular Matrix chemistry Cell culture Diffusion Chambers Culture Collagen Stress Mechanical Biomedical engineering |
| Zdroj: | ASAIO Journal. 48:8-11 |
| ISSN: | 1058-2916 |
| DOI: | 10.1097/00002480-200201000-00003 |
| Popis: | A new in vitro flow system was developed to investigate the impact of laminar flow on extracellular matrix formation and tissue development. The dynamic in vitro system was designed to provide a cross flow arrangement of main flow induced by a dialysis roller pump (500 ml/min), and nutrition flow by a perfusion pump (3 ml/hr). Poly-L-lysine precoated polyglycolic acid (PGA) scaffolds (3.14 cm2) were seeded with myofibroblasts of human aortic origin (3.0 x 10(6) cells/ mesh) and incubated for 14 days under static conditions. The tissue was exposed to shear stress over a time period of 14 days (n = 4). The control group was seeded under static conditions (n = 4). To obtain a CO2 independent medium, 25 mM HEPES and 1 mM bicarbonate buffer was supplemented to modified MEM without bicarbonate. Gas samples were collected from the medium, and hydroxyproline assay was performed as a marker of collagen production. The newly developed flow system maintained stable cell culture conditions, with the hydroxyproline concentration significantly higher in group F (p < 0.05). These preliminary experiences with a new in vitro tissue culture system demonstrate the feasibility of using flow induced mechanical stress to enhance extracellular matrix formation. |
| Databáze: | OpenAIRE |
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