Therapeutical effects of vaccine from Trypanosoma cruzi amastigote surface protein 2 by simultaneous inoculation with live parasites
| Autor: | Alexandre V. Machado, Oscar Bruna-Romero, Daniel Araki Ribeiro, Camila Pontes, Flavia de Oliveira, José Ronnie Vasconcelos, Hananiah Tardivo Quintana, Flávia Andressa Pidone Ribeiro |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Protozoan Vaccines Trypanosoma cruzi Neuraminidase medicine.disease_cause Parasitemia Vaccines Attenuated Biochemistry law.invention 03 medical and health sciences Mice 0302 clinical medicine law medicine Animals Chagas Disease Myocytes Cardiac Amastigote Molecular Biology Plaque-forming unit biology Cell Biology biology.organism_classification Molecular biology Nitric oxide synthase Oxidative Stress 030104 developmental biology Matrix Metalloproteinase 9 030220 oncology & carcinogenesis biology.protein Recombinant DNA Matrix Metalloproteinase 2 Tumor necrosis factor alpha Female Immunization Intramuscular injection Oxidative stress |
| Zdroj: | Journal of cellular biochemistry. 120(3) |
| ISSN: | 1097-4644 |
| Popis: | The aim of this study was to evaluate the efficacy of vaccine using replication-deficient human recombinant Type 5 replication-defective adenoviruses (AdHu5) carrying sequences of the amastigote surface protein 2 (ASP2) (AdASP2) in mice infected with the Trypanosoma cruzi ( T cruzi) Y strain. A total of 16 A/Sn mice female were distributed into four groups, as follows (n = 4 per group): Group 1 - Control Group (CTRL); Group 2 - Infected Group (TC): animals were infected by subcutaneous route with 150 bloodstream trypomastigotes of T cruzi Y strain; Group 3 - Immunized Group (AdASP-2): animals were immunized by intramuscular injection (im) route with 50 µL of AdSP-2 (2 × 10 8 plaque forming units [pfu]/cam) at day 0; Group 4-Immunized and Infected Group (AdASP-2+TC): animals were immunized by im route with 50 µL of ASP-2 (2 × 10 8 pfu/cam) and infected by T cruzi at the same day (day 0). It was observed a significant decrease of nests in the group that was immunized with AdASP-2 and infected on the same day. Tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) gene expressions showed a significant increase in the AdASP-2+TC group when compared to TC group, but it was noted that Cyclooxygenase-2 (Cox-2) was increased in TC group when compared to AdASP-2+TC group. Increase of matrix metalloproteinases-2 (MMP-2) and decrease of MMP-9 immunoexpression in the AdASP-2+TC group was noticed as well. Oxidative DNA damage was present in myocardium for AdASP-2+TC group as a result of 8-hydroxydeoxyguanosine immunoexpression. Taken together, our results highlighted an increased oxidative stress, MMP-2 activity and inflammatory host response promoted by AdASP-2 against T cruzi infection. |
| Databáze: | OpenAIRE |
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