Sequence analysis of the MYC oncogene involved in the t(8;14)(q24;q11) chromosome translocation in a human leukemia T-cell line indicates that putative regulatory regions are not altered
| Autor: | S N Finver, K Nishikura, L R Finger, F G Haluska, J Finan, P C Nowell, C M Croce |
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| Rok vydání: | 1988 |
| Předmět: |
Genetics
Leukemia Multidisciplinary Base Sequence Oncogene Sequence analysis T-Lymphocytes Molecular Sequence Data Nucleic acid sequence Locus (genetics) DNA Restriction Enzymes Exons Oncogenes Biology Molecular biology Translocation Genetic Cell Line Exon Regulatory sequence Humans Amino Acid Sequence Peptide sequence Gene Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 85:3052-3056 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | We have cloned the translocation-associated and homologous normal MYC alleles from SKW-3, a leukemia T-cell line with the t(8;14)(q24;q11) translocation, and determined the sequence of the MYC oncogene first exon and flanking 5' putative regulatory regions. S1 nuclease protection experiments utilizing a MYC first exon probe demonstrated transcriptional deregulation of the MYC gene associated with the T-cell receptor alpha locus on the 8q+ chromosome of SKW-3 cells. Nucleotide sequence analysis of the translocation-associated (8q+) MYC allele identified a single base substitution within the upstream flanking region; the homologous nontranslocated allele contained an additional substitution and a two-base deletion. None of the deletions or substitutions localized to putative 5' regulatory regions. The MYC first exon sequence was germ line in both alleles. These results demonstrate that alterations within the putative 5' MYC regulatory regions are not necessarily involved in MYC deregulation in T-cell leukemias, and they show that juxtaposition of the T-cell receptor alpha locus to a germ-line MYC oncogene results in MYC deregulation. |
| Databáze: | OpenAIRE |
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