Phenol glycosides extract of Fructus Ligustri Lucidi attenuated depressive-like behaviors by suppressing neuroinflammation in hypothalamus of mice
| Autor: | Yan Zhang, Xiao-Qiang Liang, Jia-Li Zhang, Fu-Hui Lin, Qiang Li, Ming-Chao He, Dezhi Tang, Rui Feng, Shufen Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Ligustrum Hypothalamus Inflammation Proinflammatory cytokine 03 medical and health sciences Mice 0302 clinical medicine Internal medicine medicine Animals Glycosides Neuroinflammation Chromatography High Pressure Liquid Pharmacology 0303 health sciences Microglia Chemistry Depression Plant Extracts 030302 biochemistry & molecular biology Angiotensin II Tail suspension test medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis Fruit TLR4 medicine.symptom |
| Zdroj: | Phytotherapy research : PTRREFERENCES. 34(12) |
| ISSN: | 1099-1573 |
| Popis: | Depression is partially caused by inflammation in central nervous system. This study investigated the ameliorative effects of phenol glycosides (PG) from Ligustrum lucidum Ait. (Oleaceae) on neuroinflammation and depressive-like behavior in mice hypothalamus as well as the molecular mechanism. Mice were administered with PG extract for 2 weeks prior to treatment with LPS. The mice treated with PG extract showed resistance to LPS-induced reduction in body weight and LPS-induced depressive-like behaviors shown by sucrose preference, tail suspension test, forced swimming test and open field test. LPS-induced activation of microglial cells and elevation in protein expression of inflammatory cytokines including IL-1β, RANTES and MCP-1 in hypothalamus of mice were abrogated by pre-treatment with PG extract. This extract down-regulated expression of TLR4, MyD88, NLRP3, renin and angiotensin II and decreased proportional area of Iba-1+ microglias in hypothalamus. Pre-treatment with PG extract inhibited LPS-triggered activation of CaSR/Gα11 signaling, stimulated 1-OHase expression in hypothalamus, and enhanced circulating 1,25(OH)2 D3 level. Overall, pre-treatment with PG extract ameliorated LPS-induced depressive-like behaviors by repressing neuroinflammation in mice hypothalamus which was attributed to its suppression on activation of microglia and production of inflammatory cytokines via acting on TLR4 pathway, CaSR and RAS cascade associated with improving vitamin D metabolism. |
| Databáze: | OpenAIRE |
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