Experimental study of histological changes in vascular loops according to the duration of the postoperative period: Application in reconstructive microsurgery
Autor: | Teng Hsiang-Wei, André Araújo Ribeiro, Rames Mattar-Jr, Renee Zon Filippe, Marcelo Rosa de Rezende, Bruno Alves Rudelli, Gustavo Bispo dos Santos, Renata Gregorio Paulos, Ana Katherina Abarca Herrera |
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Rok vydání: | 2017 |
Předmět: |
Male
Microsurgery medicine.medical_specialty Time Factors Intimal hyperplasia Neovascularization Physiologic Free flap Vascular Remodeling 030204 cardiovascular system & hematology 030230 surgery Intimal Hyperplasia 03 medical and health sciences 0302 clinical medicine Reconstructive microsurgery medicine Animals Postoperative Period Rats Wistar Vascular Loop lcsh:R5-920 business.industry Anastomosis Surgical Reproducibility of Results Arterial blood flow General Medicine Blood flow Clinical Science Hyperplasia medicine.disease Fibrosis Surgery Vascular loops Carotid Arteries Treatment Outcome Regional Blood Flow No-Reflow Phenomenon Free flap reconstruction Female Vascular Grafting Jugular Veins lcsh:Medicine (General) business |
Zdroj: | Clinics, Vol 72, Iss 9, Pp 538-542 Clinics; v. 72 n. 9 (2017); 538-542 Clinics; Vol. 72 Núm. 9 (2017); 538-542 Clinics; Vol. 72 No. 9 (2017); 538-542 Clinics Universidade de São Paulo (USP) instacron:USP Clinics, Volume: 72, Issue: 9, Pages: 538-542, Published: SEP 2017 |
ISSN: | 1807-5932 1980-5322 |
Popis: | OBJECTIVES: To analyze the histological changes observed in venous grafts subjected to arterial blood flow as a function of the duration of the postoperative period to optimize their use in free flap reconstructions. METHOD: Twenty-five rats (7 females and 18 males) underwent surgery. Surgeries were performed on one animal per week. Five weeks after the first surgery, the same five animals were subjected to an additional surgery to assess the presence or absence of blood flow through the vascular loop, and samples were collected for histological analysis. This cycle was performed five times. RESULTS: Of the rats euthanized four to five weeks after the first surgery, no blood flow was observed through the graft in 80% of the cases. In the group euthanized three weeks after the first surgery, no blood flow was observed in 20% of the cases. In the groups euthanized one to two weeks after the first surgery, blood flow through the vascular loop was observed in all animals. Moreover, intimal proliferation tended to increase with the duration of the postoperative period. Two weeks after surgery, intimal proliferation increased slightly, whereas strong intimal proliferation was observed in all rats evaluated five weeks after surgery. CONCLUSION: Intimal proliferation was the most significant change noted in venous grafts as a function of the duration of the postoperative period and was directly correlated with graft occlusion. In cases in which vascular loops are required during free flap reconstruction, both procedures should preferably be performed during the same surgery. |
Databáze: | OpenAIRE |
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