Modulation of the classical multidrug resistance (MDR) phenotype by RNA interference (RNAi)
| Autor: | Christiane Nieth, Hermann Lage, Axel Priebsch, Alexandra Stege |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Small interfering RNA
Daunorubicin Genetic enhancement Biophysics MDR1 P-glycoprotein Biochemistry Gene therapy Structural Biology RNA interference Stomach Neoplasms Pancreatic cancer Genetics medicine Tumor Cells Cultured polycyclic compounds Humans RNA Messenger RNA Neoplasm RNA Small Interfering Molecular Biology Messenger RNA biology Carcinoma Cell Biology medicine.disease Molecular biology Multiple drug resistance Gene Expression Regulation Neoplastic Pancreatic Neoplasms Phenotype biology.protein Genes MDR Gastric cancer medicine.drug |
| Zdroj: | FEBS Letters. (2-3):144-150 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(03)00523-4 |
| Popis: | For reversal of MDR1 gene-dependent multidrug resistance (MDR), two small interfering RNA (siRNA) constructs were designed to inhibit MDR1 expression by RNA interference. SiRNA duplexes were used to treat human pancreatic carcinoma (EPP85-181RDB) and gastric carcinoma (EPG85-257RDB) cells. In both cellular systems, siRNAs could specifically inhibit MDR1 expression up to 91% at the mRNA and protein levels. Resistance against daunorubicin was decreased to 89% (EPP85-181RDB) or 58% (EPG85-257RDB). The data indicate that this approach may be applicable to cancer patients as a specific means to reverse tumors with a P-glycoprotein-dependent MDR phenotype back to a drug-sensitive one. |
| Databáze: | OpenAIRE |
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