Fecal indicator bacteria, fecal source tracking markers, and pathogens detected in two Hudson River tributaries
| Autor: | Amy L. Glaser, Ruth E. Richardson, Renee R. Anderson, Dan J. Shapley, Yolanda M. Brooks, Justin S. Bae, Desiree J. Sausele, Cristina P. Fernández-Baca, Catherine M. Spirito, Emma M. Mosier, Anna Hong, Laura B. Goodman, Jennifer L. Epstein |
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| Rok vydání: | 2019 |
| Předmět: |
Veterinary medicine
Environmental Engineering 0208 environmental biotechnology Indicator bacteria 02 engineering and technology 010501 environmental sciences medicine.disease_cause 01 natural sciences Feces Rivers Rotavirus Tributary medicine Escherichia coli Giardia lamblia Animals Humans Horses Source tracking Waste Management and Disposal 0105 earth and related environmental sciences Water Science and Technology Civil and Structural Engineering geography geography.geographical_feature_category biology Bacteria Ecological Modeling Water Pollution biology.organism_classification Pollution 020801 environmental engineering Enterococcus Cattle Water Microbiology Environmental Monitoring |
| Zdroj: | Water research. 171 |
| ISSN: | 1879-2448 |
| Popis: | Volunteer monitoring in the Hudson River watershed since 2012 has identified that the Wallkill River and Rondout Creek tributary complex have elevated concentrations of the fecal indicator bacteria, enterococci. Concentrations of enterococci do not provide insight into the sources of pollution and are imperfect indicators of health risks. In 2017, the regular monthly volunteer monitoring campaign for culturable enterococci at 24 sites on the Wallkill and Rondout expanded to include: (1) culturable measurements of E. coli and quantification of E. coli and Enterococcus specific markers vis nanoscale qPCR, (2) microbial source tracking (MST) assays (avian, human, bovine, and equine) via real time PCR and nanoscale qPCR, and 3) quantification of 12 gastrointestinal pathogens including viruses, bacteria, and protozoa via nanoscale qPCR. Three human associated MST markers (HumM2, HF183, and B. theta) corroborated that human pollution was present in Rondout Creek and widespread in the Wallkill River. The presence of B. theta was associated with increased concentrations of culturable E. coli. Genes for adenovirus 40 and 41 conserved region, rotavirus A NSP3, E. coli eae and stx1, and Giardia lamblia 18S rRNA were detected in >45% of samples. Abundance of rotavirus A NSP3 genes was significantly correlated to the bovine marker gene, CowM3, though wild bird sources cannot be ruled out. This is the first study to investigate potential fecal pollution sources and pathogen concentrations in Hudson tributaries during the months of peak recreational use. |
| Databáze: | OpenAIRE |
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