Expression of d -myo-Inositol-3-Phosphate Synthase in Soybean. Implications for Phytic Acid Biosynthesis
| Autor: | Carla E. Hegeman, Laura L. Good, Elizabeth A. Grabau |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary Phytic Acid Transcription Genetic Physiology Plant Science Plant Roots Gene Expression Regulation Enzymologic chemistry.chemical_compound Biosynthesis Gene Expression Regulation Plant Complementary DNA Consensus Sequence Genetics RNA Messenger Intramolecular Lyases Inositol phosphate Conserved Sequence chemistry.chemical_classification Phytic acid Base Sequence Plant Stems ATP synthase biology Myo-Inositol-1-Phosphate Synthase Reverse Transcriptase Polymerase Chain Reaction food and beverages Isoenzymes Plant Leaves Enzyme chemistry Biochemistry Glycine biology.protein Soybeans Cotyledon Research Article |
| Zdroj: | Plant Physiology. 125:1941-1948 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.125.4.1941 |
| Popis: | Phytic acid, a phosphorylated derivative ofmyo-inositol, functions as the major storage form of phosphorus in plant seeds. Myo-inositol phosphates, including phytic acid, play diverse roles in plants as signal transduction molecules, osmoprotectants, and cell wall constituents.d-myo-inositol-3-phosphate synthase (MIPS EC5.5.1.4) catalyzes the first step in de novo synthesis ofmyo-inositol. A soybean (Glycine max) MIPS cDNA (GmMIPS1) was isolated by reverse transcriptase-PCR using consensus primers designed from highly conserved regions in other plant MIPS sequences. Southern-blot analysis and database searches indicated the presence of at least four MIPS genes in the soybean genome. Northern-blot and immunoblot analyses indicated higher MIPS expression and accumulation in immature seeds than in other soybean tissues. MIPS was expressed early in the cotyledonary stage of seed development. The GmMIPS1 expression pattern suggested that it encodes a MIPS isoform that functions in seeds to generated-myo-inositol-3-phosphate as a substrate for phytic acid biosynthesis. |
| Databáze: | OpenAIRE |
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