Identification, expansion and characterization of cancer cells with stem cell properties from head and neck squamous cell carcinomas
| Autor: | Helene Fohrer-Ting, Dale W. Lewis, Hatem Kaseb, Susanne M. Gollin, Eric Lagasse |
|---|---|
| Přispěvatelé: | Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Carcinogenesis Fluorescent Antibody Technique Cell Separation Radiation Tolerance [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Mice 0302 clinical medicine Cell Self Renewal In Situ Hybridization Fluorescence ComputingMilieux_MISCELLANEOUS Aged 80 and over biology Middle Aged 3. Good health [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology Head and Neck Neoplasms 030220 oncology & carcinogenesis Carcinoma Squamous Cell Neoplastic Stem Cells Female Stem cell [SDV.CAN]Life Sciences [q-bio]/Cancer Article 03 medical and health sciences SOX2 Antigens CD Cancer stem cell Cell Line Tumor Chromosomal Instability Radioresistance Biomarkers Tumor medicine Animals Humans Aged Cell Proliferation Cluster of differentiation Squamous Cell Carcinoma of Head and Neck CD44 Feeder Cells Cell Biology medicine.disease Head and neck squamous-cell carcinoma Clone Cells stomatognathic diseases 030104 developmental biology Cancer cell Immunology biology.protein Cancer research |
| Zdroj: | Experimental Cell Research Experimental Cell Research, Elsevier, 2016, 348 (1), pp.75-86. ⟨10.1016/j.yexcr.2016.09.003⟩ Experimental Cell Research, 2016, 348 (1), pp.75-86. ⟨10.1016/j.yexcr.2016.09.003⟩ |
| ISSN: | 0014-4827 1090-2422 |
| DOI: | 10.1016/j.yexcr.2016.09.003⟩ |
| Popis: | Head and neck squamous cell carcinoma (HNSCC) is a major public health concern. Recent data indicate the presence of cancer stem cells (CSC) in many solid tumors, including HNSCC. Here, we assessed the stem cell (SC) characteristics, including cell surface markers, radioresistance, chromosomal instability, and in vivo tumorigenic capacity of CSC isolated from HNSCC patient specimens. We show that spheroid enrichment of CSC from early and short-term HNSCC cell cultures was associated with increased expression of CD44, CD133, SOX2 and BMI1 compared with normal oral epithelial cells. On immunophenotyping, five of 12 SC/CSC markers were homogenously expressed in all tumor cultures, while one of 12 was negative, four of 12 showed variable expression, and two of the 12 were expressed heterogeneously. We showed that irradiated CSCs survived and retained their self-renewal capacity across different ionizing radiation (IR) regimens. Fluorescence in situ hybridization (FISH) analyses of parental and clonally-derived tumor cells revealed different chromosome copy numbers from cell to cell, suggesting the presence of chromosomal instability in HNSCC CSC. Further, our in vitro and in vivo mouse engraftment studies suggest that CD44+/CD66− is a promising, consistent biomarker combination for HNSCC CSC. Overall, our findings add further evidence to the proposed role of HNSCC CSCs in therapeutic resistance. |
| Databáze: | OpenAIRE |
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