Nicotine promotes the intracellular growth of Mycobacterium tuberculosis in epithelial cells
| Autor: | Claudia Valdez Miramontes, Jeny de Haro Acosta, Adrián Rodríguez-Carlos, Bruno Rivas-Santiago, Sara P. Marin-Luevano, Luis A. Martinez, José Antonio Enciso-Moreno |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Nicotine beta-Defensins Tuberculosis alpha7 Nicotinic Acetylcholine Receptor medicine.medical_treatment 030106 microbiology Immunology Antimicrobial peptides Pharmacology Microbiology Cathelicidin Mycobacterium tuberculosis 03 medical and health sciences Cathelicidins medicine Humans Nicotinic Agonists Receptor Tuberculosis Pulmonary biology Chemistry Macrophages medicine.disease biology.organism_classification Bacterial Load Nicotinic acetylcholine receptor 030104 developmental biology Infectious Diseases A549 Cells Alveolar Epithelial Cells Host-Pathogen Interactions Intracellular Antimicrobial Cationic Peptides medicine.drug |
| Zdroj: | Tuberculosis. 127:102026 |
| ISSN: | 1472-9792 |
| DOI: | 10.1016/j.tube.2020.102026 |
| Popis: | Several epidemiological studies have identified the cigarette smoke as a risk factor for the infection and development of tuberculosis. Nicotine is considered the main immunomodulatory molecule of the cigarette. In the present study, we evaluated the effect of nicotine in the growth of M. tuberculosis. Lung epithelial cells and macrophages were infected with M. tuberculosis and/or treated with nicotine. The results show that nicotine increased the growth of M. tuberculosis mainly in type II pneumocytes (T2P) but not in airway basal epithelial cells nor macrophages. Further, it was observed that nicotine decreased the production of β-defensin-2, β-defensin-3, and the cathelicidin LL-37 in all the evaluated cells at 24 and 72 h post-infection. The modulation of the expression of antimicrobial peptides appears to be partially mediated by the nicotinic acetylcholine receptor α7 since the blockade of this receptor partially reverted the production of antimicrobial peptides. In summary, it was found that nicotine decreases the production of HBD-2, HBD-3, and LL-37 in T2P during the infection with M. tuberculosis promoting its intracellular growth. |
| Databáze: | OpenAIRE |
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