cAMP regulates the functional activity, coupling efficiency and structural organization of mammalian FOF1 ATP synthase
| Autor: | Loris Micelli, Arcangela Santeramo, Sergio Papa, Domenico De Rasmo, Paolo Lattanzio, Anna Signorile |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Biophysics Oxidative phosphorylation Mitochondrion Biology Biochemistry Oxidative Phosphorylation Myoblasts 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate Cyclic AMP Myocyte Animals Inner mitochondrial membrane Cells Cultured Membrane potential Adenosine Triphosphatases Membrane Potential Mitochondrial 030102 biochemistry & molecular biology ATP synthase Mitochondria FOF1 ATP synthase Intramitochondrial cAMP sAC Membrane Proteins Cell Biology Mitochondrial Proton-Translocating ATPases Cell biology Rats 030104 developmental biology chemistry Membrane protein biology.protein Carrier Proteins Adenosine triphosphate Adenylyl Cyclases |
| Zdroj: | Biochimica et biophysica acta. Bioenergetics (2016). doi:10.1016/j.bbabio.2016.01.006 info:cnr-pdr/source/autori:De Rasmo D, Micelli L, Santeramo A, Signorile A, Lattanzio P, Papa S./titolo:cAMP regulates the functional activity, coupling efficiency and structural organization of mammalian FOF1ATP synthase/doi:10.1016%2Fj.bbabio.2016.01.006/rivista:Biochimica et biophysica acta. Bioenergetics/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| ISSN: | 0006-3002 |
| DOI: | 10.1016/j.bbabio.2016.01.006 |
| Popis: | The present study shows that in isolated mitochondria and myoblast cultures depletion of cAMP, induced by sAC inhibition, depresses both ATP synthesis and hydrolysis by the FOF1 ATP synthase (complex V) of the oxidative phosphorylation system (OXPHOS). These effects are accompanied by the decrease of the respiratory membrane potential, decreased level of FOF1 connecting subunits and depressed oligomerization of the complex. All these effects of sAC inhibition are prevented by the addition of the membrane-permeant 8-Br-cAMP. These results show, for the first time, that cAMP promotes ATP production by complex V and prevents, at the same time, its detour to a mitochondrial membrane leak conductance, which is involved in cell death. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|