Eucalyptus microcorys leaf extract derived HPLC-fraction reduces the viability of MIA PaCa-2 cells by inducing apoptosis and arresting cell cycle
| Autor: | Christopher J. Scarlett, Michael C. Bowyer, Quan V. Vuong, Anita C. Chalmers, Danielle R. Bond, Deep Jyoti Bhuyan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Cell cycle checkpoint Cell Survival Apoptosis Pharmacology Antioxidants Eucalyptus microcorys 03 medical and health sciences 0302 clinical medicine food Cell Line Tumor Pancreatic cancer medicine Humans Cytotoxicity Chromatography High Pressure Liquid Cell Proliferation Chromatography Reverse-Phase Eucalyptus biology Plant Extracts Chemistry Cell Cycle Microcorys Cell Cycle Checkpoints General Medicine Cell cycle medicine.disease biology.organism_classification Pancreatic Neoplasms Plant Leaves 030104 developmental biology Phytochemical 030220 oncology & carcinogenesis Apoptosis Regulatory Proteins |
| Zdroj: | Biomedicine & Pharmacotherapy. 105:449-460 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2018.05.150 |
| Popis: | New therapeutic strategies such as the development of novel drugs and combinatorial therapies with existing chemotherapeutic agents are urgently needed to improve the clinical prognosis of pancreatic cancer. We have previously reported the antiproliferative properties of aqueous crude Eucalyptus microcorys extract against pancreatic cancer cell lines. In this study, bioassay-guided fractionation of the aqueous crude E. microcorys extract using RP-HPLC and subsequent assessment of the resultant fractions (F1-F5) for their antioxidant activity and cytotoxicity against pancreatic cancer cell lines were performed. The molecular mechanisms associated with the cytotoxicity was characterised by studying the effects of the most potent fraction-1 (F1) on apoptosis and cell cycle profiles as well as its phytochemical constituents by LC-ESI/MS/MS. F1 displayed significantly greater antioxidant activity in three different assays (p 0.05). Moreover, F1 exhibited significantly greater antiproliferative activity (IC |
| Databáze: | OpenAIRE |
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