Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy
| Autor: | Volha Malechka, Yusuke Takahashi, Younghwa Shin, Jian Xing Ma, Gennadiy Moiseyev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Rhodopsin genetic structures medicine.drug_class Retinoic acid Biology Article Retina Pathology and Forensic Medicine Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound Internal medicine Diabetes mellitus medicine Animals Visual Pathways Retinoid Rats Wistar Type 1 diabetes Diabetic Retinopathy Diabetic retinopathy medicine.disease Retinol binding protein Disease Models Animal 030104 developmental biology Endocrinology chemistry biology.protein Retinaldehyde sense organs Retinol-Binding Proteins Plasma Visual phototransduction Photoreceptor Cells Vertebrate |
| Zdroj: | The American Journal of Pathology |
| ISSN: | 1525-2191 0002-9440 |
| Popis: | Diabetic retinopathy is a common complication of diabetes mellitus. Diabetic patients experience functional deficits in dark adaptation, contrast sensitivity, and color perception before microvascular pathologies become apparent. Herein, we evaluated early changes in neural retinal function and in retinoid metabolism in the eye in diabetes. Streptozotocin-induced diabetic rats showed decreased a- and b-wave amplitudes of scotopic and photopic electroretinography responses 4 months after diabetes induction compared to nondiabetic controls. Although Western blot analysis revealed no difference in opsin expression, rhodopsin content was decreased in diabetic retinas, as shown by a difference in absorbance. Consistently, levels of 11-cis-retinal, the chromophore for visual pigments, were significantly lower in diabetic retinas compared to those in controls, suggesting a retinoid deficiency. Among visual cycle proteins, interphotoreceptor retinoid-binding protein and stimulated by retinoic acid 6 protein showed significantly lower levels in diabetic rats than those in nondiabetic controls. Similarly, serum levels of retinol-binding protein 4 and retinoids were significantly lower in diabetic rats. Overall, these results suggest that retinoid metabolism in the eye is impaired in type 1 diabetes, which leads to deficient generation of visual pigments and neural retinal dysfunction in early diabetes. |
| Databáze: | OpenAIRE |
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