Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli

Autor:	Bruggeman, Christine W., Haasnoot, Guus H., Peterman, Erwin J.G.
Přispěvatelé:	Physics of Living Systems, LaserLaB - Molecular Biophysics
Rok vydání:	2023
Předmět:	Microscopy Model Organisms General Immunology and Microbiology General Neuroscience Biophysics Cell Biology SDG 6 - Clean Water and Sanitation General Biochemistry Genetics and Molecular Biology
Zdroj:	STAR Protocols, 4(1):102121. Elsevier Bruggeman, C W, Haasnoot, G H & Peterman, E J G 2023, ' Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli ', STAR Protocols, vol. 4, no. 1, 102121, pp. 1012 . https://doi.org/10.1016/j.xpro.2023.102121
ISSN:	2666-1667
Popis:	Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and imaging of C. elegans through fluorescence microscopy to determine their molecular and/or cellular response to chemosensory stimuli. This protocol can also be applied to amphid neurons by inserting the worm in the chip head-first. For complete details on the use and execution of this protocol, please refer to Bruggeman et al. (2022).1
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f404d87d4e1c869fcdad463f61f21987 https://doi.org/10.1016/j.xpro.2023.102121 Zobrazit plný text záznamu