Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase

Autor:	Tom Beneke, Ulrich Dobramysl, Carolina Moura Costa Catta-Preta, Jeremy Charles Mottram, Eva Gluenz, Richard Wheeler
Rok vydání:	2023
Předmět:	570 Life sciences biology Medicine (miscellaneous) General Biochemistry Genetics and Molecular Biology 570 Biowissenschaften Biologie
Zdroj:	Beneke, Tom; Dobramysl, Ulrich; Catta-Preta, Carolina Moura Costa; Mottram, Jeremy Charles; Gluenz, Eva; Wheeler, Richard J (2022). Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase. Wellcome open research, 7(294), p. 294. Wellcome Trust 10.12688/wellcomeopenres.18575.1
ISSN:	2398-502X
DOI:	10.12688/wellcomeopenres.18575.2
Popis:	We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3f5c6857819a61e17d8393cf6bf4e78 https://doi.org/10.12688/wellcomeopenres.18575.2 Zobrazit plný text záznamu