Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues
| Autor: | Ana Chueca, Pablo Pulido, Florence Vignols, Francisco Javier Cejudo, Antonio Jesús Serrato, Mariam Sahrawy, Yves Meyer, José A. Traverso, Roland Cazalis |
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| Přispěvatelé: | Estación Experimental del Zaidín (EEZ), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Umea Plant Science Center (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU)-Swedish University of Agricultural Sciences (SLU), Universidad de Sevilla |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Physiology [SDV]Life Sciences [q-bio] Mutant Gene Expression Plant Science Biology 01 natural sciences Malate dehydrogenase Pisum 03 medical and health sciences Thioredoxins Yeasts Vascular cambium Protein Isoforms ComputingMilieux_MISCELLANEOUS Vascular tissue 030304 developmental biology 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction Genetic Complementation Test Peas food and beverages Hydrogen Peroxide biology.organism_classification Complementation Chloroplast Oxidative Stress Biochemistry Multigene Family Thioredoxin Plant Structures 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2008, 59 (6), pp.1267-1277. ⟨10.1093/jxb/ern037⟩ |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Plants are the organisms containing the most complex multigenic family for thioredoxins (TRX). Several types of TRXs are targeted to chloroplasts, which have been classified into four subgroups: m, f, x, and y. Among them, TRXs f and m were the first plastidial TRXs characterized, and their function as redox modulators of enzymes involved in carbon assimilation in the chloroplast has been well-established. Both TRXs, f and m, were named according to their ability to reduce plastidial fructose-1,6-bisphosphatase (FBPase) and malate dehydrogenase (MDH), respectively. Evidence is presented here based on the immunocytochemistry of the localization of f and m-type TRXs from Pisum sativum in non-photosynthetic tissues. Both TRXs showed a different spatial pattern. Whilst PsTRXm was localized to vascular tissues of all the organs analysed (leaves, stems, and roots), PsTRXf was localized to more specific cells next to xylem vessels and vascular cambium. Heterologous complementation analysis of the yeast mutant EMY63, deficient in both yeast TRXs, by the pea plastidial TRXs suggests that PsTRXm, but not PsTRXf, is involved in the mechanism of reactive oxygen species (ROS) detoxification. In agreement with this function, the PsTRXm gene was induced in roots of pea plants in response to hydrogen peroxide. © 2008 The Author(s). |
| Databáze: | OpenAIRE |
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