Long term exposure to cadmium: Pathological effects on kidney tubules cells in Sparus aurata juveniles

Autor: Monica Tizzano, Raffaele Panzuto, Raimondo Cerciello, Palma Simoniello, Bice Avallone, Rosaria Scudiero, Chiara Maria Motta, Maria Rosa Montinari, Patrizia Cretì, Carmela Pizzoleo
Přispěvatelé: Avallone, Bice, Cerciello, Raimondo, Cretì, Patrizia, Pizzoleo, Carmela, Scudiero, Rosaria, Tizzano, Monica, Panzuto, Raffaele, Simoniello, Palma, Montinari, Maria Rosa, Motta, CHIARA MARIA, Avallone, B, Cerciello, R, Cretì, P, Pizzoleo, C, Scudiero, R, Tizzano, M, Panzuto, R, Simoniello, P, Montinari, Mr, Motta, Cm
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
Brush border
Health
Toxicology and Mutagenesis
Lectin staining
chemistry.chemical_element
Cell Count
Chemical
010501 environmental sciences
Cadmium chloride
Biology
Aquatic Science
Kidney
01 natural sciences
Inactivation
Glycocalyx
03 medical and health sciences
chemistry.chemical_compound
Cadmium Chloride
Internal medicine
medicine
Metallothionein
Animals
Brush border
Cadmium
Kidney tubule cells ultrastructure
Lectin staining
Metallothionein
PAS staining
Water Pollutants
Toxicology and Mutagenesis
0105 earth and related environmental sciences
Cadmium
PAS staining
Kidney tubule cells ultrastructure
Inactivation
Metabolic
Kidney Tubules
Sea Bream
Water Pollutants
Chemical
Staining
030104 developmental biology
Endocrinology
medicine.anatomical_structure
chemistry
Health
Ultrastructure
Metabolic
Popis: The effects of an exposure to cadmium chloride 0.47 μM for 150 days were studied in kidneys of juveniles Sparus aurata by a multidisciplinary approach so to correlate uptake and detoxification potential to changes in brush border and glycocalyx sugar composition. Results demonstrated that cadmium concentration in kidney significantly increased from day 30 reaching a plateau on day 120 while metallothioneins reached a peak on day 90 and by day 120 were already decreasing to control values. Cytological damage was extensive on day 90, clearly detectable at both structural and ultrastructural levels, in tubular cells and brush-border. Staining with a panel of four lectins revealed a significant increase in N-Ac-Gal and a decrease in mannose in the glycocalyx and the tubular basal membranes. From day 120, when cadmium concentration was high and metallothionein concentration decreasing, a clear recovery was observed in tubular cells morphology and sugar composition. Possible significance of these apparently contrasting data are discussed.
Databáze: OpenAIRE
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